Chronological gene expression profile in acute renal failure using CDNA array method

1.利用低循环RT-PCR技术建立了非RI cDNA芯片方法。2.建立了人近端上皮细胞体外缺氧复氧模型。3.建立小鼠肾缺血再灌注模型作为体内模型; 4.利用非RI cDNA芯片获得缺氧/复氧可逆/不可逆损伤的基因表达谱; 5.重点研究可逆期表达增加和不可逆损伤减少的基因亚群。作为体外模型中预测可逆性的标志基因6.在体内模型中，关注在可逆期增加表达和减少不可逆损伤的基因亚组作为预测可逆性的标志基因。

1.Non-RI CDNA array method was established utilizing low cycle RT-PCR.2.In vitro hypoxia reoxyganetion model was established using human proximal epithelial cells. Time points that distinguish reversible from irreversible damage was determined.3.Mice renal ischemia-reperfusion model was established as in vivo model.4.Gene profiles in reversible/irreversible damage from hypoxia reoxyganetion were obtained using non-RI CDNA array.5.Subgroup of genes that increase expression during reversible phase and decrease in irreversible damage are focused-as the marker genes prophetic of reversibility in in vitro model.6.Subgroup of genes that increase expression during reversible phase and decrease in irreversible damage are focused as the marker genes prophetic of reversibility in in vivo model.