The Role of Tissue Factor (TF) In the development of Ischemic-reperfusion injury in Organ Transplantation

(背景)。组织因子（Tissue factor， TF）是血液凝固的起始因子，在炎症或免疫反应过程中在内皮细胞上诱导表达。我们设计了一种反义寡脱氧核苷酸（AS-1/TF），并研究了其对大鼠肝缺血再灌注损伤的影响（方法）。AS-1/TF经静脉给药予Lewis大鼠。10小时后，部分夹住肝动脉和门静脉。180分钟后肝脏再灌注，取肝。免疫组化染色检测TF表达（结果）。10只大鼠中有1只存活5天，未治疗组内皮细胞上有强烈的TF染色。然而，经AS-1/TF处理，7只中有6只存活，TF染色明显降低。此外，我们观察到荧光蛋白标记的AS-1/TF被内皮细胞吸收。上述结果提示，AS-1/TF能强烈抑制TF的表达，从而抑制大鼠肝脏缺血再灌注损伤

(Background.) Tissue factor (TF) is an initiation factor for blood coagulation, and its expression is induced on endothelial cells during inflammatory or immune responses. We designed an antisense oligodeoxynucleotide (AS-1/TF) for rat TF, and studied its effect on hepatic ischemic reperfusion injury(Methods.) AS-1/TF was delivered intravenously to Lewis rats. After 10 hours, hepatic artery and portal vein were partially clamped. Livers were reperfused after 180 minutes and were harvested. TF expression was studied using immunohistochemical staining(Result.) One of 10 rats survived in a 5-day survival rates and TF was strongly stained on endothelial cells in nontrearment group. However, by treatment with AS-1/TF, six of seven survived and TF staining was significantly reduced. Furthermore, we observed that fluoroscein labeled AS-1/TF was absorbed into endothelial cells.(Conclusions.) These results suggest that AS-1/TF can strongly suppress the expression of TF and thereby inhibit ischemic reperfusion injury to the rat liver