Analysis using DNA chip for responder, and non-responder in patients with advanced esophageal cancer

目的：晚期食管癌患者中应答相关基因的研究尚不清楚。食管癌患者放化疗疗效相关基因的检测是食管癌放化疗疗效评价的重要依据。方法：对23例食管癌患者进行内镜下标本采集，并对采集的标本进行分析。使用激光捕获显微切割方法（PixCell II LCM系统，Arcturus Inc.）从癌细胞中提取RNA。通过线性扩增法（Rikagaku Research Center）扩增足够的mRNA。应用cDNA微阵列（Rikagaku Research Center，人类20K芯片）对23例食管癌患者的正常食管鳞状细胞进行基因筛选。结果：应用该技术未发现与食管癌放化疗相关的基因。因此，我们不能在大会上展示这些结果，也不能将这些结果提交给期刊。结论：食管癌放化疗疗效相关基因的分析是食管癌放化疗疗效相关基因研究的迫切需要。然而，许多机构尚未获得任何临床使用的有用信息。最近，据说食管癌的放疗和手术切除一样有效。因此，这种治疗在未来将更加相关。此外，为了临床应用，有必要检测与反应相关的基因。这项研究将有助于改善食道癌患者的治疗结果。

Purpose : The responder-related genes for in patients with advanced esophageal cancer remain unclear. It is essential to detect responder or non-responder related genes for chemoradiation in patients with esophageal cancer.Methods : Specimens were obtained endoscopically in 23 patients, who gave informed consent for this research, with advanced esophageal cancer. RNA was extracted from cancer cells using laser capture microdissection method (PixCell II LCM system, Arcturus Inc.). Sufficient mRNA was amplified by the linear amplification method (Rikagaku Research Center). Screening of genes was performed using cDNA microarray (Rikagaku Research Center, human 20K chip) in 23 patients with esophageal cancer reffering normal esophageal squamous cell. The correlation was evaluated between screened genes and clinicopathological outcomes (imaging response, histological response, survival).Results : Any relatied-genes responsible for chemoradiation of esophageal cancer were not found with this technique. Therefore, we could not present these results in a congress or submit these results to a journal. Inappropriate procedure of RNA extraction or insufficient dose of chemoradation may cause these results.Conclusions : Analysis of related-genes for responder or non-responder for chemoradiation of esophageal cancer is urgent. However, any useful information for clinical use has not been obtained in many institutions. Recently, chemoradiation for esophageal cancer is said to be effective as well as surgical resection. Therefore, this treatment will be more relevant in the future. Moreover, it is necessary to detect the response related-genes for clinical use. This research will contribute to improve therapeutic outcomes in patients with esophageal cancer.