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cDNA microarray analyses on the gene expression in the pituitary glands under the physiological and experimental conditions.

cDNA微阵列分析生理和实验条件下垂体的基因表达。

基本信息

批准号：
15590152
负责人：
WATANABE Tsuyoshi
金额：
$ 2.43万
依托单位：
Asahikawa Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

The pituitary gland is situated in the center of hypothalamic-pituitray-gonadal axis that cooperatively regulates the reproductive functions. Pituitary gonadotropes regulate the gonadal function by secreting two gonadotropins, LH and FSH, and in turn, their functional states are influenced by the hypothalamic and gonadal factors. To describe the molecular basis of these influences on the pituitary gland, we analyzed simultaneously the expression levels of 11,505 genes in the rat pituitary and adrenal glands under the physiological and experimental conditions by using a cDNA microarray.First, we found significant sex-related differences in the expression levels of 112 genes in the pituitary and 87 genes in the adrenal glands. Interestingly, only 4 genes of the 112 genes identified above in the pituitary exhibited simultaneously a significant sex-related difference in the expression in the adrenal, suggesting that the molecular basis for the sex-related differences varies depending on th … More e tissues/cells. These sex-related differences in the gene expression are significantly reduced after castration (orchiectomy for male, and ovariectomy for female rats), indicating that the gonadal factors play a pivotal role in the establishment of sex-related differeneces in these two organs. However, the gene expression of these two endocrine organs were differently regulated by androgens and estrogens ; the expression levels of about three fourths of the 112 genes identified above in the pituitary were significantly fluctuated by estradiol treatment after castration in correspondence with the sex-related difference observed, while those of four fifths of the 87 genes identified above in the adrenal were significantly fluctuated by testosterone treatment.These analyses first clarified the molecular basis of sex-related differences in the two representative organs, pituitary and adrenal glands. Based on these findings, the gene products that have not been classified so far could be identified as a molecule with a significant function in the hypothalamic-pituitary-gonadal axis. Less

垂体位于下丘脑-垂体-性腺轴的中枢，协同调节生殖功能。垂体促性腺激素通过分泌两种促性腺激素LH和FSH调节性腺功能，反过来，它们的功能状态受下丘脑和性腺因素的影响。为了阐明这些影响垂体的分子基础，我们使用cDNA微阵列同时分析了生理和实验条件下大鼠垂体和肾上腺中11，505个基因的表达水平。首先，我们发现垂体中112个基因和肾上腺中87个基因的表达水平存在显著的性别差异。有趣的是，在垂体中鉴定的112个基因中，只有4个基因在肾上腺中的表达同时表现出显著的性别相关差异，这表明性别相关差异的分子基础取决于性别。 ...更多信息 e组织/细胞。去势（雄性大鼠睾丸切除，雌性大鼠卵巢切除）后，这些与性别相关的基因表达差异显著减少，表明性腺因素在这两个器官中建立与性别相关的差异中起着关键作用。但这两个内分泌器官的基因表达受雄激素和雌激素的调控不同;垂体中上述鉴定的112个基因中约四分之三的表达水平在去势后通过雌二醇处理显著波动，这与观察到的性别相关差异一致，而肾上腺中上述87个基因中的五分之四的基因因睾酮治疗而显着波动。这些分析首次阐明了性的分子基础-两个代表性器官，垂体和肾上腺的相关差异。基于这些发现，迄今尚未分类的基因产物可以被鉴定为在下丘脑-垂体-性腺轴中具有重要功能的分子。少