Study for the mechanism of the development of co-lateral vein in portal hypertension

5周龄同系BL/6小鼠作为Lac-Z转基因小鼠的供体。采用900 cOy全身照射后行骨髓移植。OCL 4诱导肝纤维化。本实验采用肝细胞生长因子（HGF）基因转移技术，观察骨髓细胞在肝再生过程中是否能被转移到肝脏内。轻度损伤的肝再生中肝细胞或卵圆细胞均不来源于造血干细胞。将造血干细胞输送到内皮细胞后，重建肝脏。本实验中同侧静脉发育，但未见造血细胞，不能说明门静脉高压症同侧静脉发育的机制。

Five-week-old syngeneic BL/6 mouse served as donors for Lac-Z transgenic mouse. Bone marrow transplantation was performed.After whole body irradiation with 900cOy. Fibrotic liverwas induced by administration of OCL4. Hepatocyle growth factor(HGF) gene transfer was performed and examined if bone marrow cell would be delivered to liver or not in regeneration of liver.We got the following finds. Hepatocytes or oval cells did not derive from hematopoietic stem cell in hepatic regeneration in a little damaged liver. After hematopoietic stem cell was delivered to the endothelial cell, the liver was reconstructed. In this experiment, the co-lateral vein developed but hematopoietic cell was not found. We could not show the mechanism of the development of co-lateral vein in portal hypertension.