Genetic informations of gliomas by whole-genome analysis using genome microarray

我们试图用基因组微阵列来定义胶质瘤的遗传信息。[方法]采用比较基因组杂交（CGH）技术检测了包括37例胶质母细胞瘤在内的52例胶质瘤的DNA拷贝数畸变（CNAs），并通过19例胶质母细胞瘤的DNA微阵列分析进一步验证了CNAs的增加。[结果]在所有37个样本中都检测到CNAs，主要是缺失，并且经常涉及染色体lp (35%), 10 (54%), 19q（38%）和22q（49%）。CNAs的增加经常涉及染色体7（16%）和8q（11%）。人胶质母细胞瘤的基因组畸变主要是缺失和少量扩增，在新生型中常见。在19例病例中，使用GenoSensor System （Vysis, Inc., Downers Grove， IL， USA）对58个致癌基因（AmpliOne I）进行DNA微阵列分析。EGFR （7pl2）扩增6例（31%），PDGFRA （4ql2）扩增2例（11%），MYC（8ql2）扩增1例（5%），CCND/FGF4扩增1例（1lq3）。这些扩增相对较强，范围从4倍到32倍。7号染色体的数据与基因芯片的数据不一致。[结论]微阵列系统可方便、快速地从临床样品中获取遗传信息，不仅可用于诊断，而且可用于有序的治疗。

We attempted to define the genetic informations of gliomas using genome microsrray.[Methods] The DNA copy number aberrations (CNAs) were detected by comparative genomic hybridization (CGH) in 52 gliomas including 37 glioblastomas and the observed gains in CNAs were further verified by DNA microarray analysis in 19 glioblastomas.[Results] CNAs were detectable in all of the 37 samples analyzed and were mainly deletions and frequently involved Chromosomes lp (in 35% instances), 10 (54%), 19q (38%), and 22q (49%.). Gains in CNAs were frequently involved Chromosomes 7 (16%) and 8q (11%).Genomic aberrations in human glioblastomas were deletions mainly and a few amplifications which were seen frequently in de novo type. In 19 cases DNA microarray analysis was performed against 58 oncogenes (AmpliOne I) using GenoSensor System (Vysis, Inc., Downers Grove, IL, USA). Amplification of EGFR (7pl2) were seen in 6 cases (31%), PDGFRA (4ql2) in 2 case (11%), MYC(8ql2) in 1 case (5%), and CCND/FGF4 (1 lq3) in one case. These amplifications were relatively strong ranged from 4 times to 32 times. The date about chromosome 7 from CGH and genome microarray was inconincidence. [Conclusion] It is apparent to get genetic informations casily and rapidly from clinical samples using microarray system It must be used not only in diagnosis but in order made therapy.