Effect of HMG CoA reductase inhibitor on kidney allograft function

本研究探讨了氟伐他汀（FV）在体外有丝分裂原诱导或同种异体淋巴细胞增殖反应中的潜在作用。<Materials and methods>将人外周血淋巴细胞与I12、PHA或同种异体淋巴细胞在微量培养板上共培养。将FV以2、10、50、100和150 ng/ml的终浓度施用至每个孔。计数3 H标记的胸苷的摄取以估计淋巴细胞增殖。<Results>FV对IL 2诱导的淋巴细胞增殖无抑制作用，CPM值分别为6942.7 ± 912.2（FV=2ng/ml）、8493.7 ± 1967.3（FV= 10 ng/ml）、6482 ± 3126.7（FV= 50 ng/ml）、7976.3 ± 2063.9（FV= 100 ng/ml）、3903 ± 2591.4（FV= 150 ng/ml）。FV对PHA诱导的淋巴细胞增殖无抑制作用，CPM值分别为26930 ± 11448.6（FV=0），31048.33 ± 7619.4（FV=2），25309.7 ± 9573.4（FV=10），31613 ± 8745.9（FV=50），23643.3 ± 4605.2（FV=100），14290.3 ± 8405.4（FV=150）。FV对同种异体刺激试验无抑制作用（应答者A对失活刺激物B），CPM值为9848.3 ± 4580.3（FV=0）、107640 ± 3659.3（FV=2）、10898.7 ± 888.3（FV=10）、7599.3 ± 1084.7（FV=50）、5140.3 ± 501.4（FV=100），7257.7 ± 3945.9（FV=150），而FV&gt; 50 ng/ml显著抑制应答者B对灭活刺激物A的不同组合中的同种异体刺激试验，CPM值分别为16202.2 ± 2733.8（FV=0），17952.3 ± 5363.5（FV=2），13898.3 ± 3033.6（FV=10），9169 ± 3902.9（FV=50）、8170.3 ± 2598.9（FV=100）、7981.3 ± 2481.7（FV=150）。<Conclusion>FV对丝裂原诱导的淋巴细胞增殖无抑制作用，但对同种异体淋巴细胞刺激有抑制作用。

We investigated the potential role of fluvastatin(FV) in mitogen induced or allogeneic lymphocyte proliferative reactions in vitro.<Materials and methods> Human peripheral blood lymphocytes were co-cultured with I12, PHA, or allogeneic lymphocytes in microcultureplate. FV was administered to each well at a final concentration of 2, 10, 50, 100 and 150ng/ml. The uptake of 3H-labeled thymidine was counted to estimate lymphocyte proliferation.<Results> FV did not suppress the IL2 induced lymphocyte proliferation with the CPM value of 6942.7 ± 912.2(FV=2ng/ml), 8493.7 ± 1967.3(FV=10ng/ml), 6482 ± 3126.7(FV=50ng/ml), 7976.3 ± 2063.9(FV=100ng/ml), 3903 ± 2591.4(FV=150ng/ml). FV did not suppress the PHA induced lymphocyte proliferation with the CPM value of 26930 ± 11448.6(FV=0), 31048.33 ± 7619.4(FV=2), 25309.7 ± 9573.4(FV=10), 31613 ± 8745.9(FV=50), 23643.3 ± 4605.2(FV=100), 14290.3 ± 8405.4(FV=150). FV did not suppress alogeneic stimulation test (responder A against inactivated stimulator B) with the CPM value of 9848.3 ± 4580.3(FV=0), 107640 ± 3659.3(FV=2), 10898.7 ± 888.3(FV=10), 7599.3 ± 1084.7(FV=50), 5140.3 ± 501.4(FV=100), 7257.7 ± 3945.9(FV=150), however, FV more than 50 ng/ml significantly suppressed the allogeneic stimulation test in a different combination of responder B against inactivated stimulator A with CPM value of 16202.2 ± 2733.8(FV=0), 17952.3 ± 5363.5(FV=2), 13898.3 ± 3033.6(FV=10), 9169 ± 3902.9(FV=50), 8170.3 ± 2598.9(FV=100), 7981.3 ± 2481.7(FV=150). <Conclusion> FV did not suppress the mitogen induced lymphocyte proliferation, however, may have suppressive effect on allogeneic lymphocyte stimulation.