The basic study concerning the high pregnancy rate by in vitro fertilization-embryo transfer treatment in infertile women with antisperm antibody

CBA/J雌性小鼠与C57BL/6雄性小鼠交配。在阴道涂片中检测到精子的时间被指定为怀孕0.5天。从未怀孕和怀孕的小鼠身上切除子宫。将污染的DNA消化后，提取总RNA，逆转录。以3-磷酸甘油醛脱氢酶(GAPDHmRNA)的表达水平为内标，用Taq Man化学方法进行定量聚合酶链式反应。在妊娠1.5d(0.71±-0.31)~2.5d(22.55+/-10.69)，子宫组织中降钙素/GAPDHmRNA的比值显著升高，在第35d(111.38+/-120.92)达到高峰，到4.5d下降到21.54+/-3.65。降钙素基因在妊娠7.5天(21.12±24.19)天和9.5天(32.73±3.12)天继续表达。但在妊娠9.5天，子宫中无胚胎的部位降钙素mRNA/GAPDHmRNA的比值很低(2.71+/-1.65)，说明小鼠在着床期子宫中有降钙素mRNA的表达。此外，降钙素mRNA在妊娠7.5天和9.5天表达的结果表明，降钙素mRNA的表达也受到胚胎信号的调节。

CBA/J female mice were mated with C57BL/6 male mice. The time when the presence of sperm was detected in a vaginal smear was designated day 0.5 of gestation. The uteri were excised from nonpregnant and pregnant mice. Total RNA was isolated and reverse-transcribed after the digestion of contaminating DNA. Quantitative PCR for calcitonin mRNA was performed by using Taq Man Chemistry, and the expression level of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA was measured as an internal standard.The ratio of calcitonin mRNA/GAPDH mRNA in the uterus dramatically increased between days 1.5 (0.71 +/- 0.31) and 2.5 (22.55 +/- 10.69) of gestation, reached a peak on day 3.5 (111.38 +/- 120.92), and decreased to 21.54 +/- 3.65 on day 4.5. Calcitonin mRNA continued to be expressed on day 7.5 (21.12 +/- 24.19) and day 9.5 (32.73 +/- 3.12) of gestation. However, the ratio of calcitonin mRNA/GAPDH mRNA in the part of the uterus in which the embryo was not present was very low on day 9.5 (2.71 +/- 1.65) of gestation.These findings demonstrate that calcitonin mRNA in the uteri of mice appeared to be expressed during the implantation period. Furthermore, the findings that calcitonin mRNA was expressed on day 7.5 and day 9.5 of gestation suggest that calcitonin mRNA expression is also regulated by the embryonal signal.