The difference of the adhesion molecule system between blastocyst implantation and cancer invasion

方法. 4-用输卵管冲洗法从超数排卵并交配的ICR雌性小鼠中收集细胞期胚胎。将胚胎孵育至扩张囊胚期。根据卵裂球活检的位置将受试者分为以下亚组：A组; 180°方向周围，B组;与内细胞团（ICM）成90°方向周围，C组;靠近ICM。很少有卵裂球用“狭缝”法活检。使用抗α5β3、抗α6β4整联蛋白和抗α5β1整联蛋白抗体对这些卵裂球进行化学染色。此外，这些囊胚在活检后在培养皿上孵育72小时。测量囊胚的铺展面积，比较α5β3/α6β4整合素阳性和阴性囊胚的铺展面积。结果A、B组中有α5β3和α6β4整合素表达，C组中无表达。在任何组中均未观察到α5β3整合素的表达。α5β3/α6β4整合素阳性囊胚的扩张面积大于阴性囊胚。根据这些结果，我们得出结论，α5β3和α6β4整合素在活检的卵裂球上的表达与囊胚扩展活性密切相关。

Methods. 4-cell stage embryos were collected from superovulated and mated ICR female mice with oviduct flushing method. Embryos were incubated until expanded blastocyst stage. Subjects were classified into subgroups according to the position of blastomere biopsy as following, Group A; around the 180° direction, Group B; around the 90° direction from the inner cell mass (ICM), Group C; near ICM. Few blastomeres were biopsied with the "slit" method. These blastomeres were immunohistochemically stained using anti-α5β3, anti-α6β4 integrin and anti-α5β1 integrin antibody. Moreover, these blastocysts were incubated on culture dish for 72 hours after biopsy. The spreading areas of these blastocysts were measured, and were compared between α5β3/α6β4 integrin positive and negative one. Results Expression of α5β3 integrin and α6β4 integrin was seen in Group A and B, but was not in Group C. Expression of α5β3 integrin was not observed in any groups. The spreading area of α5β3/α6β4 integrin positive blastocyst is larger than that of negative one.Conclusions. From these results, we conclude that expression of α5β3 and α6β4 integrin on biopsied blastomeres is closely associated with blastocyst spreading activity.