The immunohistochemical, molecular biological and clinical research of MALT lymphoma in the salivary gland

研究对象为1977年1月至2001年12月间424例涎腺恶性肿瘤，均采用组织学或病理学方法。首先，重新检查这些病理报告，并选择淋巴滋生病变的报告。接下来，重新检查病理样本，并提取被认为是MALT淋巴瘤的病例。通过使用以下两种方法，在这些病例中检查单克隆性作为支持诊断。（1）使用免疫球蛋白携带细胞的各类别抗体进行染色，如果在一个类别中存在染色的极化，则判断存在单克隆性。（2）从石蜡包埋的新鲜冰冻标本中提取DNA。通过PCR扩增超变区之一的CDR3区来检测IgH重建。如果PCR产物检测为单一条带，则判定样品具有单克隆性。对诊断为MALT淋巴瘤的病例， ...更多信息 同时用免疫组化和PCR方法检测其与MALT淋巴瘤危象和EB病毒感染的关系。经复查诊断，11例16份标本诊断为MALT淋巴瘤。5例8例标本诊断为MALT淋巴瘤。MALT淋巴瘤的诊断似乎比较困难。11例MALT淋巴瘤中多合并干燥综合征，也有合并类风湿因子等自身免疫性疾病的病例。提示MALT淋巴瘤与免疫异常有关。在性别方面，11例中有7例为女性。所以，女人的比例很高。我们试图观察涎腺MALT淋巴瘤的预后，迄今为止没有死亡的例子，但是，在也认识到病变部分位于涎腺外的病例中，没有病例达到完全缓解。因此，MALT淋巴瘤的唾液腺并不总是表现出良好的进展，本研究从遗传学角度提示了MALT淋巴瘤与EB病毒感染的关系，但免疫组化检查未能证实。少

Objects are salivary gland neoplasm 424 examples of enforcing extraction or biopsy method from January, 1977 to December, 2001. First, these pathology reports were reexamined, and reports for the lymph breeding lesion were selected. Next, the pathological samples were reexamined, and the cases which were thought to be MALT lymphoma were extracted. By using two following methods, the monoclonality was examined as a support diagnosis in these cases.(1)The dyeing was carried out using each class antibody of the immunoglobulin carrying cell, and it was judged that there was monoclonality, if there is the polarization of the dyeing in one class.(2)DNA was extracted from paraffin embedding freshly frozen samples. The IgH reconstitution was examined by amplifying CDR3 region which is one of the super variable region by the polymerase chain reaction. If PCR product is detected as the single band, it was judged that the sample had the monoclonality. On the cases diagnosed as MALT lymphoma, ther … More apies, prognoses, etc. were examined.Additionally, the relevance to MALT lymphoma crisis and EB(Ebstein-Barr) virus infection was examined by immunohistochemical and PCR method. As a result of the review diagnosis, 11 cases, 16 samples were diagnosed as MALT lymphoma. It was 5 cases, 8 samples that the diagnosis were changed to MALT lymphoma. It seemed that the diagnosis of MALT lymphoma was difficult. The Sjogren syndrome was combined in most of 11 cases diagnosed as MALT lymphoma, and there were cases with autoimmune diseases such as rheumatoid factor. These facts showed that MALT lymphoma has the relation with the immunological abnormality. And, in the sex, there were 7 woman examples in 11 examples. So, there was the high proportion of the woman. We tried to observe the prognosis of the salivary gland of MALT lymphoma and it was recognized that there was no examples of death until now However, in the cases which also recognized the lesioned part place in the salivary gland outside, there is no cases which came to the complete remission. So, the salivary gland of MALT lymphoma did not always show the good progress.The relation between MALT lymphoma and EB virus infection was indicated from the genetics examination in this study, though the proof was not obtained from the immunohistochemical examination. Less