Apoptosis-promoting gene Bax transfer potentiates themosensitivity of squamous cell caruroma I vitro

为探讨bax基因表达在细胞凋亡相关机制中对热敏性的调控作用，本研究以携带bax基因的哺乳动物表达载体转染人头颈部癌细胞（IMC-3），检测其加热后的热敏性和细胞凋亡。用克隆法测定细胞热敏性。根据凋亡细胞的发生、DNA的断裂和聚（adp -核糖）聚合酶（PARP）的裂解来评估细胞凋亡。与亲本细胞相比，IMC-3细胞中bax基因的过表达增强了热敏性。与IMC-3细胞相比，bax转染IMC-3细胞诱导细胞凋亡、DNA断裂和PARP裂解更为明显。这些发现表明bax通过增强诱导haat诱导的细胞凋亡来促进热敏化。

To investigate the role of bax gene expression in modulating thermosensitivity through an apoptosis-related mechanism, we examined the thermosensitivity and apoptosis after heating in a human head and neck cancer cells (IMC-3 cell lines) transfected with bax gene-bearing mammalian expression vector. Cellular thermosensitivity was determined using the clonogenic assay. Apoptosis was assessed on the basis of the occurrence of apoptotic cells, DNA fragmentation and poly(ADP-ribose)polymerase (PARP) cleavage. Overexpression of the bax gene in IMC-3 cells enhanced thermosensitivity compared with parent cells. The induction of apoptotic cells, DNA fragmentation and PARP cleavage in bax transfected IMC-3 cells were more prominent than those in IMC-3 cells. These findings suggest that bax contributes to thermosensitization by enhancing the induction of haat-induced apoptosis.