Intracellular Ca2+ mobilization and measurements of nitric oxide synthesis on cultured nasal microvascular endothelial cells

除了培养人鼻粘膜血管内皮细胞外，我们还回顾了手术时获得的人鼻粘膜血管内皮细胞培养体系的建立和上皮纤毛运动的影响，但支持细胞的建立困难，在培养细胞内纤毛运动的测量不成功。结合我们新购买的图像分析设备和电子闪光灯复制，回顾了目前的测量系统。正在研究中，并用组胺和白甲苯负荷量在Fura-2同时测定细胞内钙水平的实验系统中利用已有的血管内皮细胞和DAF-2上一年的细胞内NO生成量进行了实验。在我们的实验系统中，培养的血管内皮细胞在组胺负荷下细胞内钙水平的升高，揭示了…较多的NO产生，而它是从负载直接返回，因为它通过了负载超过10分钟。NO的产生与浓度的依赖关系尚不清楚。白质激素也有类似的趋势。预先在培养液中加入脂多糖(LPS)可促进NO的产生，并提示iNOS的表现。从这些检查中可以看出，鼻粘膜血管内皮细胞产生NO的能力对鼻黏膜微循环有贡献，至于变应性鼻炎出现鼻塞，血管内皮细胞紊乱导致的NO产生能力下降似乎是原因之一。纤毛上皮细胞的功能受NO的调控，至于表皮细胞产生的NO，则提示血管内皮细胞是通过内毒素刺激产生的剪应力而产生的。本研究认为，这既产生了鼻黏膜呼出物中的NO，又决定了两者之间的相互关系及与临床病情的关系。较少

Besides culture human nasal mucosa vascular endothelial cells, we reviewed the influence of the nitric oxide to the establishment of the culture system of the human nasal mucosa vascular endothelial cells which we obtained at the time of surgery and epithelium ciliary movement, but the establishment of supporting cells is difficult and does not succeed in the measurement of the ciliary movement in the cultured cell.It combines the image analysis device which we purchased newly with electronic flashes copying and reviews the measurement system now.It was under study, and histamin and the leukotoluene load carried out an experiment in the experiment system of the same time measurement of calcium level in cells by fura-2 using the vascular endothelial cells which there was and the quantity of NO production in cells by DAF-2 in last year. As for the elevation of the intracellular calcium level of cultured vascular endothelial cells in the histamine load in our experiment system, it reveale … More d the NO production whereas it was found from the load direct back since it passed after load more than 10 minutes. The concentration-dependency was not clear about the NO production. An approximately similar tendency was present about the leukotoluene.The NO production when we added lipopolysaccharide (LPS) in culture medium beforehand was enhanced, and manifestation of iNOS was suggested..From these examinations, NO of nasal mucosa vascular endothelial cells-producing ability contributed to the microcirculation of the nasal mucosa, and, as for the nasal congestion to be found in allergic rhinitis, it seemed that fall of the NO production ability by the vascular endothelial cells disorder was one of cause. The function of the ciliated epithelium is controlled from a past report by NO, and, as for the NO, that epidermic cells produce is suggested vascular endothelial cells by shearing stress by LPS stimulation. It was thought that this both produced the NO derived from a nasal mucosa excreted by exhalation and determined these mutual relations and connection with the clinical condition in this study. Less