Study of skin reconstruction by using the technique of tissue engineering

本研究将纤维蛋白与其他纤维蛋白结合，设计组织工程皮肤。角化细胞在纤维蛋白凝胶基质上培养，易分泌蛋白酶消化纤维蛋白凝胶。因此，纤维蛋白凝胶基质作为支架的机械强度是不稳定的。当一种可溶性结缔组织材料（专利申请中）混合在纤维蛋白凝胶基质中时，角质形成细胞的蛋白酶消化受到保护。此外，利用新型纤维蛋白支架可以在凝胶基质中培养真皮成纤维细胞，然后在凝胶基质上培养角质形成细胞。从这些结果来看，这种新型纤维蛋白支架可能是一种有用的装置，如组织工程皮肤。现在我们尝试将新型支架应用于这种组织工程皮肤的临床应用。

In this investigation, tissue engineered skin was designed by using the fibrin and other one. Keratinocytes were cultured onto the fibrin gel matrix but keratinocytes secreted protease digested fibrin gel easily. Therefore, the mechanical strength of fibrin gel matrix as the scaffold was labile. When one soluble connective tissue material (patent pending) was mixed in the fibrin gel matrix, the protease digestion by keratinocytes was protected. Furthermore, by using the new type fibrin scaffold, dermal fibroblasts could be cultured in gel matrix and then keratinocytes onto the gel matirix. From these results, this new type fibrin scaffold might be useful device such as tissue-engineered skin. Now we attempted to apply to this tissue-engineered skin by using new type scaffold clinically.