Exhaustive analysis of gene expression dining bone regeneration

我们进行了以下实验。1.本实验首先在生长期小鼠股骨干上制作直径为2.2mm的圆孔，观察骨髓间充质干细胞在骨修复过程中的增殖活性，并观察BrdU掺入情况。这些研究表明，在我们的实验系统中，损伤后3天，增殖的含BrdU的间充质细胞增加.骨再生过程中基因表达的变化利用上述实验模型，我们通过北方印迹分析研究了骨相关基因表达模式的变化。骨损伤后第4天ALP mRNA升高，第5天骨钙素mRNA升高。组织化学和免疫组织化学研究表明这些分子的表达模式相似.上述实验表明，在我们的实验模型中，间充质干细胞在损伤后4天和5天出现。我们正在利用cDNA芯片探索间充质干细胞的相关基因。

We conducted the following; experiments.1. Characterizaiion of mecenchymal stem cells during bone regenerationWe first made n round hole, 2.2 mm in a diameter, at diaphysis of femurs of growing mice To Investigate the proliferation activity of mesenchymal cells during the bone repair, we explored incorporation of BrdU. These studies demonstrated that proliferating mesenchymal cells incorporating BrdU increased 3 days after injury in our experimental system.2. Changes in gene expression during bone regenerationUsing above described experimental model, we investigated the changes in expression patterns of bone-related genes by Northern blot analysis. ALP mRNA increased on day 4 and osteocalcin mRNA increased OB day 5 after bone injury. Histochemical and immunohistochemical studies indicated similar expression patterns of these molecules.3. Above experiments suggested that mesenchymal stem cells appeared during days 4 and 5 after injury-in our experimental model. We are now exploring the genes related to mesenchymal stem cells by cDNA chips.