IMPROVEMENT AND DEVELOPMENT FOR IMPLANT MATERIALS USING HUMAN GINGIVAL EPITHELIAL CELLS

1991年，石川从人牙龈建立了人上皮细胞系，并将其命名为HGE-15。由于我们已经使用HGE-15细胞研究了各种生物材料的生物相容性。2000年至2001年的调查结果如下：1. 2000年，我们主要研究了ADVANCE公司提供的高结晶度涂层羟基磷灰石（HA）的生物相容性，从而在早期阶段给出了其表面结构与其他涂层HA相比具有优势的新认识.在2001年，我们增加了一些其他的生物材料（商业钛，HA冲击钛，多孔HA，高速框架HA，等离子HA，和Falcon），并研究了使用HGE-15细胞和MC 3 T3细胞和人骨细胞作为相同的实验系统。虽然我们目前已经分析了结果，但我们已经了解到，各种生物材料的生物相容性因细胞类型而异。此外，我们和HA材料的生物相容性较差， ...更多信息 本文通过透射电镜（TEM）、扫描电镜（SEM）等方法，对钛和Falcon的作用机理进行了研究，并在第七届仿生工程：陶瓷的新作用（6月14日，意大利Faenza）的特邀演讲中部分报道了这些结果。此外，目前，我已经进行了合作和开发，并调查了新的生物材料，提供了NIRIN和国家陶瓷技术研究所意大利国家研究理事会的生物学研究。大多数以前的细胞培养研究牙龈上皮细胞和各种生物材料的附着主要使用原代细胞，然而，这些细胞和生物材料之间的粘附是不够的。1991年，石川等人提出SGL细胞虽然具有牙龈上皮细胞的特性，但其是否具有与牙龈上皮细胞相同的特性仍存在疑问，并建立了人牙龈上皮细胞系，该体系和结果对体内和临床上上皮细胞与各种生物材料的粘附机制提供了许多建议。少

In 1991, Ishikawa established a human epithelial cell line from human gingiva and named it HGE-15. Since we have studied the biocompatibility for various biomaterials using HGE-15 cells. The results of this from 2000 to 2001 are as follows ;1. In 2000, we have investigated mainly the biocompatibility the coating hydroxyapatie (HA) indicated high crystallization offered from ADVANCE Co. ltd. As a result, we have given a new knowledge that its surface structure has advantage compared with other coating HA in early stage.2. In 2001, we have added some other biomaterials (commercial titanium, HA impacted titanium, porous HA, high-velocity frame HA, plasma HA, and Falcon) and studied ones using HGE-15 cells and MC3T3 cells and human bone cells as same experimental system. Though we have analyzed the results at present, we have understood that the biocompatibility for various biomaterials has differed by using cell type. Furthermore, we and the biocompatibility of HA materials are poor compa … More red with the titanium and Falcon, and start the cell adhesion to adsorb some protein in high-velocity frame HA in early stage etc by transmission electron microscopy (TEM) and scanning electron microscopy (SEM).I have partly reported these results in invited lecture at the 7th Biomimetic Engineering ; A new Role for Ceramics (June, 14 Faenza, Italy). Furthermore, At present, I have carried out the cooperation and development and investigated the biological studies of the new biomaterials that offered NIRIN and National Institute for Ceramic Technology Italian National Research Council.Five studies have examined human gingival cells in vitro as present. Most previous cell culture studies regarding the attachment of gingival epithelial cells and various biomaterials have used mainly primary cells ; however, adhesion between these cells and biomaterials are insufficient. In 1991, Ishikawa claimed that although SGL cells, it remains questionable whether they have the same properties as gingival epithelial cells and newly established a human gingival epithelial cell line.This system and results should provide a lot of suggestion with regard to the mechanism by which epithelial cells adhere to various biomaterials in vivo and clinic. Less