Apoptosis induces of a multi-drug resistant oral cancer cells by Tax01, Alkaroid derived ICHI

【目的】对在众多恶性肿瘤中占绝大多数的鳞状细胞癌进行了常规化疗、放疗、手术治疗等综合治疗。然而，你不应该进行令人满意的医疗结果。此外，高频使用的化疗药物表明口服域鳞状细胞癌存在耐药的癌细胞，据说对一种化疗药物耐药的恶性肿瘤具有所谓的交叉耐药能力，即对其他化疗药物耐药。近年来，紫杉醇被发现是一种新的抗肿瘤药物。紫杉醇是由ICHII科药材半复配而成的生物碱，是一种不同于常规抗肿瘤药物的作用机制。本研究比较了紫杉醇对口腔癌多药耐药细胞的抗肿瘤作用。此外，还可以报道一种多重耐药癌症的耐药机制。【结果和结论】——紫杉醇对我们实验室建立的NA-CDDP细胞和NA-ADM细胞没有交叉抗性。紫杉醇对CDDP和ADM耐药的病例也可能有作用，因此临床上应用紫杉醇。此外，MRP-2/cMOAT在NA-CDDP细胞中的表达量是NA细胞的4倍左右，提示MRP-2/cMOAT显著参与了CDDP耐药。另一方面，由于NA-CDDP细胞MRP-3基因的表达量约为NA细胞的12倍，因此通过MRP-2/cMOAT提示，MRP-3对CDDP的细胞外转运能力并没有提高。总之，紫杉醇已成为治疗多药耐药口腔癌的有效药物。此外，通过研究多药耐药癌细胞的耐药机制，有可能有助于药物敏感性的诊断。少

[purpose]Medical treatment by combined use of the conventional chemotheraoy, radiotherapy, a surgery treatment, etc. has been performed to the squampus cell carcinoma which has the large majority of many malignant tumor. However, you should not carry out satisfactory of the medical treatment results. Moreover, existence of the cancer cell which shows reresitance is indicated to oral domain squamous cell carcinoma by the chemotherapeutic drug used tor high frequency, and it is said that the malignant tumor which shows resistance to one chemotherapeutic drug has the so-called cross resistance ability which shows resistance to other chemotherapeutic drugs.Recently, Taxol was found out as a new anti-neoplasm agent. Taxol is the alkaloid half-compounded from the department medicinal herb of ICHII, and is a different mechanism foreword from the conventional antineoplastic drug. In this study, comparison of Taxol's anti-neoplasm effect of multi drug resistance of the oral cancer cells establi … More shed by our laboratory in serum-free culture. Moreover, the resistance mechanism of a multi drug-resistant cancer can is reported.[result and conclusion]--Taxol did not show cross resistance to NA-CDDP cells and NA-ADM cells established by our laboratory. A possibility that Taxol expressed an effect was suggested also to the case which shows resistance to CDDP and ADM by which the present clinical application is carried out. Moreover, the amount of expression of MRP-2/cMOAT in NA-CDDP cells is the amount of expression of the amount of about 4 times as compared with NA cells, and it was suggested that MRP-2/cMOAT is participating in CDDP resistance significantly. On the other hand, since there was about 12-time amount of expression of MRP-3 gene of NA-CDDP cells as compared with NA cells, the rather than thing which the transportation-cell outside ability of CDDP through MRP-3 is going up was suggested through MRP-2/cMOAT. In conclusion, it was thought that Taxol became an effective cure to a multi-drug resistance oral cancer. Moreover, it was possible by solving the resistance mechanism of a multi drug-resistant cancer cell to contribute to diagnosis of medicine susceptibility. Less