Prevention of aphtous stomatitis with α-linolenic acid -a double-blind study-

选取43名每月至少发生1次口腔溃疡的受试者（男11名，女32名;平均年龄43岁），采用双盲试验方法，对紫苏油的预防效果进行了研究。在前4个月，要求所有受试者使用色拉油（大豆油和菜籽油的50-50混合物）作为公鸡油，以控制研究受试者的起始条件（导入期）。他们还被要求减少他们的公鸡油消耗。在导入期结束时，他们被随机分配到紫苏油组或对照组。在紫苏油组中，他们使用紫苏油作为食用油8个月，不使用其他食用油（实验期）。对照组只使用大豆油。在整个实验期间（12个月），受试者被要求在我们提供的日历上标记他们患口疮性口炎的日子，紫苏组和对照组之间的红细胞磷脂组分的脂肪酸组成没有显著差异。两组口腔炎的发生率也无显著差异。然而，如果将两组合并，口腔炎的总数显著减少（导入期为2.8±1.3/月，实验期为2.0±1.3/月，p<0.0001）。发病率降低的原因尚不清楚，但可能有以下原因：（1）实验期间未使用的菜籽油是口腔炎的原因，（2）两组中均存在安慰剂效应。

Forty-three subjects (11 males and 32 females; the mean age: 43 years) who suffered from aphthous stomatitis at least once a month were recruited, and the preventive effects of perilla oil were tested in a double-blind study. For the first 4 months, all the subjects were asked to use salad oil (a 50-50 mixture of soybean oil and rapeseed oil) as cocking oil in order to control the starting conditions of the study subjects (run-in period). Also they were asked to reduce their cocking oil consumption. At the end of the run-in period, they were randomly assigned either to a perilla oil group or a control group. In the perilla oil group they used perilla oil as a cooking oil for 8 months and not to use other cooking oils (experimental period). In the control group they used soybean oil only. For the whole experimental period (12 months), subjects were asked to mark the days when they suffered from aphthous stomatitis on the calendar we provided.There were no significant differences in the fatty acid composition of the RBC phospholipid fraction between the perilla and control groups. There were no significant differences in the incidence of stomatitis between the two groups, either. However, if the two groups were combined, the total number of stomatitis was significantly reduced (2.8±1.3/month in the run-in period to 2.0±1.3/month in the experimental period, p<0.0001). The reason why the incidence was reduced was not clear, but the followings might be possible: (1) Rapeseed oil, which was not used in the experimental period, was responsible for stomatitis, and (2) there were placebo effects in both groups.