Molecular diversity and emergence of environmental adaptation of Cucumber mosaic virus in Southeast Asia

黄瓜花叶病毒(CMV)寄主范围广，危害严重。然而，在大多数关于CMV的研究中，日本和欧洲等温带地区的分离物是人们感兴趣的对象，对热带地区CMV的研究还不够充分。本研究以几个来自东南亚的分离物为研究对象，对甜瓜抗CMV品种进行了检测，对来自印度尼西亚的CMV分离物进行了分子分析，对来自印度尼西亚的CMV卫星RNA进行了序列分析，并对来自菲律宾、印度尼西亚和日本的分离物进行了系统发育分析。因此，由于CMV抗性基因尚未见报道，因此发现了一个抗CMV的甜瓜抗CMV家族，并建立了检测CMV抗性的特异标记。本研究以香蕉等植物为代表的单子叶植物和番茄植物为研究对象，获得了几个分离物。对从香蕉植株上检测到的CMV病毒基因组RNA的部分序列进行了分析，并比较了它们对不同香蕉品种的反应。从番茄中检测到CMV卫星RNA。在对印尼番茄田和这些卫星的调查中，RNAS可能是东南亚的第一份报告。将它们的特定序列与其他报道的CMV卫星进行了比较。根据系统发育分析，Palkaitis等人将5株毒株分为II亚群，5株毒株划分为亚洲株。由于需要进一步收集东南亚地区的CMV，因此本研究认识到东南亚或热带地区CMV的多样性及其在抗病作物和弱毒品种选育中的重要意义。

Cucumber mosaic virus (CMV) has a wide host range and causes serious diseases in many agricultural crops. In most of the studies on CMV, however, isolates from temperate zone such as Japan and European countries were targets of interests and researches on CMVs from tropic area are not fully studied. In this study, detection of CMV resistant Cucumis melo varieties, molecular analysis of CMV isolates from bananas in Indonesia, sequence analysis of CMV satellite RNAs from Indonesia, and phylogenic analysis of CMV isolates from the Philippines, Indonesia and Japan were conducted mainly with several isolates from Southeast Asia. As the results, a family of CMV resistant C.melo was found since CMV resistant genes have not been reported, and specific markers to detect CMV resistance were developed. Banana and other plants in genus Musa as the representative of monocotyledonous plants and tomato plants were focused in this study and several isolates were obtained. Partial sequences of virus genomic RNAs from CMVs detected in banana plants were analyzed and compared their reaction on different banana varieties. From tomatoes, CMV satellite RNAs were detected. in the survey of Indonesian tomato fields and these satellite RNAS could be the first report in Southeast Asia. Their specific sequences were compared with other reported CMV satellites. Based upon phylogenic analysis, 5 isolates classified into Subgroup II serologically and 5 isolates into Asian strains by Palkaitis et al. Since further collection of CMV in Southeast Asia is required, the diversity of CMV in Southeast Asia or tropic zone and their significance in development of CMV resistant crop and selection of attenuated CMV were recognized in this study.