Experimental study of generation mechanism of thinner abuser's sudden death: Molecular biological examination of myocardial damage

父亲阿布瑟突然死亡的原因可能还不确定。观察甲苯吸入对心脏器官的影响。雄性Wistar大鼠吸入甲苯(1500ppm，每天4小时)，连续7天。病理上观察心脏，未见明显的冠状动脉闭塞、狭窄等异常。异常清楚，如血管壁、血管内皮细胞和冠状动脉狭窄。未见心肌紊乱，间质未见细胞浸润。传导系统也未观察到明显的异常，如细胞侵入。免疫组织化学染色氧化损伤标志物。DNA氧化损伤，如8-OH-DG已被承认，但脂质过氧化的形成，如4-HNE尚不清楚。免疫组织化学染色显示吸入组小血管呈免疫阳性反应。对于这一结果，血液中超氧化物歧化酶的循环供应(达到)被认为是超氧化物歧化酶的出现。经8-OH-DG定量分析，对照组为1.150[+！-]0.096，治疗组为1.073[+！-]0.119 ng/ml。这一结果与免疫组织化学染色结果有很好的一致性。因此，DNE氧化损伤的发生尚不能得到定量证实。提出了由于抗氧化剂作用的小血管中出现了超氧化物歧化酶，从而控制了甲苯对8-羟基-dG的出现加重的可能性。甲苯直接对心脏造成过氧化损伤，或甲苯吸入过量产生肾上腺皮质激素引起心脏过氧化损伤。因此，提出了影响心功能的可能性。

The cause of fhinner abouser's sudden death might be uncertain. The effect of toluene inhalation on heart organs was examined. Male Wistar rats was inhaled toluene (1,500 ppm for 4 hours a day) for 7 days. Pathologically the heart was observed, therefor there was no clear anomalous such as the obturations and the strictures in the coronary aretery. Anomalous clear as for the vessel wall and the endothelial and the strictures in the coronary artery. The myocardial disarray was not observed, and the cell infiltration was not observed in the interstitum. Clear anomalous such as cell invasions was not observed even by the conduction system either. Then, immunohistochemically markers of oxidative damage were stained. The oxidative DNA damage, such as 8-OH-Dg was admitted, although the formation of lipidperoxide, such as 4-HNE was not clear. The immunohistochemical staining of SOD revealed that immunopositivity in the small vessels of the inhalation group. As for this result, the supply of circulation of the blood SOD (attainment) to the heart was thought to the appearance of SOD. By quantitative analysis of 8- OH-Dg, it was 1.150【+!-】0.096 in the control and 1.073【+!-】0.119 in ng/ml in treated group. This result was corresponding to the immunohistochemical staining result well. Therefore, the generation of the oxidative DNE injury was not able to be confirmed quantitatively. The possibility that the appearance accentuation of 8-OH-Dg by the toluene is controlled since the appearance of SOD in the small vessel acted anti-oxidativelly was suggested. The peroxidative damage is caused to the heart directly by the toluene, or the possibility to couse the heart the peroxidation damage through an excessive production of the adrencorticotropine by the toluene inhalation is suggestion. As a result, the possibility to influence the cardiac function was suggested.