The relationship between Helicobacter pylori infection and polymorphism of immune-related gene

我们于2001年4月至2004年3月进行了一项病例对照研究，以阐明幽门螺杆菌感染的形成与免疫相关基因多态性之间的关系。研究参与者都是健康的日本人，他们每年都参加体检。本研究所有受试者均获得书面知情同意，并经岛根大学伦理委员会批准。我们选择了96名幽门螺杆菌血清阳性和96名幽门螺杆菌血清阴性的年龄和性别匹配的受试者。从白细胞中提取基因组DNA，分析肿瘤坏死因子(TNF)-α、IL-10、cd14启动子区（-308）和甘露糖结合凝集素（MBL）两个位点的基因组多态性。因此，我们在本研究中调查的五种免疫相关基因的多态性在幽门螺杆菌血清阳性和血清阴性受试者之间没有观察到有意义的差异。因此，我们认为遗传免疫机制的差异可能不会影响幽门螺杆菌感染的形成。在我们进行这项研究期间，我们澄清了长期幽门螺杆菌感染可降低血清高密度脂蛋白胆固醇水平。然而，动脉硬化的程度受年龄的影响，而不受幽门螺杆菌感染的影响。此外，血清同型半胱氨酸水平在幽门螺杆菌血清阳性和阴性个体之间没有差异。我们在英国医学杂志上报道了这些发现。

We performed a case-control study to clarify the relationship between the formation of Helicobacter pylori (H. pylori) infection and polymorphisms of immune-related gene from April 2001 to March 2004. Study participants were healthy Japanese, who attended for annual medical check-up. written informed consents were obtained by all subjects for this study, which was approved by ethical committee of Shimane University. we selected 96 subjects with seropositive of H. pylori and age-and sex-matched 96 subjects with seronegative of H. pylori. Genomic DNA was extracted from white blood cell and analyzed the genomic polymorphisms in promotor region (-308) of tumor necrosis factor (TNF)-α, IL-10, CD 14 and two sites of Mannose-binding lectin (MBL). As a result, meaningful differences in polymorphism of five immune-related genes, we investigated in this study, were not observed between H pylori-seropositive and seronegative subjects. Therefore, we concluded that the difference in hereditary immune mechanism may not influence the formation of H. pylori infect ion.During we performed this study, we clarified chat long-term H. pylori infect ion reduces serum level of high-density lipoprotein cholesterol. However, the degree of arteriosclerosis is influenced by aging and not by H. pylori infect ion. In addition, serum homocysteine level is not different between H. pylori-seropositive and negative individuals. We reported these findings in English medical journal.