DEVELOPMENT OF NRORQPROTECTIVE STRATEGY BY THE COMBIMTIQN THERAPY OF MILD HYPOTHERMIA AND NEURQNAL NITRIC OXIDE INHIBITOR ADMINISTRATION AGAINST NEONATAL BRAIN DAMAGE

本研究的目标是通过研究临床神经保护策略的组合疗法的意义是中等hypothermia和神经元氮氧化物抑制剂（7-硝基咪唑）管理局对低氧化学（HI）脑损伤对新生儿颜料的影响。我们利用HI新生脑损伤模型研究了不同模式冷却的影响，使用Cerebral和系统血动力学对新生脑损伤模型。新生猪，年龄不到两天，已被分析、半分析和机械化。我们不断地测量的皮质电泳谱图（EEG） ，通过近红外光谱仪测量的cerebral oxygenation index （TOI），通过激光多普勒流量测量，平均动脉血压力（MABP） ，心率（HR），周边动脉血氧饱和度（Sp 02） ， rectal and cerebral （nasopharyngeal）温度。我们还可以测量血气和血糖。这些颜料被提交给了普通的胡萝卜素的临时分布和 ... More hypoxemia （Fi 02 ^0. 15，呼吸率： 15节拍/分钟），为60分钟。那只小猪被释放的胡萝卜素，并提供的Fi 02。每分钟0次和60次呼吸的呼吸率。猪被分配到选择性脑冷却（自然药理学温度; 35）。51，温度; 38。5吨，24小时），全身冷却（天然气和放射性温度; 35。5 ^C，24小时）或正常母亲（自然生长和放射性温度; 38。5-C）很快就会恢复。在那之后，冷却的猪在1点上缓慢地重新变暖。0-C每小时，其温度在正常温度范围内。HI在TOI、rCBF、Sp 02、EEG振幅中的恶化表明，在基线后返回到所有颜料的恢复。EEG参数was then observed to be gradually decreasing （like the delayed "secondary" energy failure）。Nasopharyngeal的温度被0度降低。5~1. 0 C in the selective brain cooling。rCBF、TOI、Sp 02、EEG振幅、MABP、HR、血胶和血液葡萄糖在选择性和全身冷却中的两个冷却和再升温阶段之间没有明显的差异，并与选择性热蛋白炎之间的这些参数进行比较。在冷却和冷却阶段有整体冷却和整体冷却，在冷却和冷却阶段表明整个身体冷却是不重要的反向效应，与选择性大脑冷却相比较。Less（低）

The goal of this study was to investigate the clinical neuroprotective strategy by means of the combination therapy of mild hypothermia and the neuronal nitric oxide inhibitor (7-nitroindazole) administration against hypoxic-ischemic (HI) brain damage in neonatal piglets. We investigated the effects of the different mode of cooling on the cerebral and systemic hemodynamics using HI neonatal brain damage models.Neonatal piglets, aged less than two days, were anesthetized, paralyzed and mechanically ventilated. We continuously measured cortical electroencephalogram (EEG) , cerebral oxygenation index (TOI) by near-infrared spectroscopy, regional cerebral blood flow (rCBF) by laser Doppler flowmetry, mean arterial blood pressure (MABP) , heart rate (HR), peripheral arterial blood oxygen saturation (Sp02) , rectal and cerebral (nasopharyngeal) temperatures. We also measured blood gases and blood glucoses. The piglets were subjected to temporary occulusion of the common carotid arteries and … More hypoxemia (Fi02^0. 15, respiratory rate: 15 beaths/min) for 60 min. The piglets were then resuscitated by releasing the carotid occluders and providing the Fi02 of 1. 0 and respiratory rate of 60 breaths/min. Piglets were divided into either the selective brain cooling (nasopharyngeal temperature; 35. 51, ectal temperature; 38. 5 t for 24 hours), whole-body cooling (nasopharyngeal and rectal temperatures; 35. 5 ^C, for 24 hours) or the normothermia (nasopharyngeal and rectal temperatures; 38. 5 ーC) soon after resuscitation. Thereafter, the cooled piglets were slowly rewarmed at 1. 0 ーC per hour until their temperature was within the normal temperature range.HI insult indicated decreases in TOI, rCBF, Sp02, EEG amplitude, which were returned to baseline after resuscitation in all piglets. The EEG amplitude was then observed to be gradually decreasing (like the delayed "secondary" energy failure). The nasopharyngeal temperature was decreased by 0. 5~1. 0 "C in the selective brain cooling. There were no significant differences in rCBF, TOI, Sp02, EEG amplitude, MABP, HR, blood gase, and blood glucose during cooling and rewarming phase in both selective and whole-body cooling, compared with no were no significant differences in these parameters between the selective rmo bra thermia. There in cooling and whole-body cooling during cooling and rewarming phase, which indicated that the adverse effects in the whole-body cooling were not significant, compared with selective brain cooling. Less