Experimental and clinical study of the treatment by acupuncture and moxibustion on the postoperative complaints after digestive surgery

(1)为研究电针对大鼠小肠运动的影响，采用应变式力传感器法连续记录大鼠胃肠运动，并观察了植物神经调节药物和电针对空肠运动的影响。电刺激腹部引起与新斯的明给药相同的反应，腹部刺激后不规则收缩持续5 h，后肢电针后1 ~ 3 h内消化间期肌电复合波（IMC）频率增加。(2)为阐明电针对大鼠结肠运动的影响机制及胆囊收缩素（CCK）在其中的调节作用，本实验观察了中枢CCK受体的参与及脑脊液（CSF）中CCK免疫反应性的变化。结果表明，高频电针可诱导脑脊液中CCK的大量释放，通过激活中枢CCK-A受体促进结肠运动。(3)为探讨针刺内关穴对视动性运动病的影响，对每位受试者进行运动病评分和胃电图（EGG）变化分析。受试者暴露于旋转视动鼓以诱导向量。在旋转暴露之前、期间和之后记录EGG。留针组运动病的主要症状之一恶心的严重程度较非留针组明显减轻，胃电活动正常带的百分比较非留针组明显增加。从这些结果可以认为，留针刺激的PC6穴位是有效的视动性运动病可能是通过抑制异常的胃肌电活动。

(1) To study the effect of electrical acupuncture (EA) on intestinal motility, we employed the strain gauge force transducer method to record the gastrointestinal motility of rats continuously and investigated the effects of drugs acting on the autonomic nervous function and EA on jejunal motility. Electrical stimulation to the abdomen caused the same reaction as neostigmin administration and irregular contractions continued for 5 hours after abdominal stimulation, and the frequency of IMC (Interdigestive myoelectric complex) increased during 1 to 3 hours after EA to the hind limbs.(2) To elucidate the mechanism of the effects of the EA colonic motility in rats and the role of cholecystokinin (CCK) in regulating them, the participation of the central CCK receptors and the changes of CCK-immunoreactivities in cerebrospinal fluid (CSF) were investigated. The results indicated that high frequency EA induces a significant release of CCK in CSF, which accelerate the colonic motility through the activation of central CCK-A receptors.(3) To investigate the effect of acupuncture stimulation at PC6 (Neiguan) on optokinetic motion sickness, motion sickness scores were evaluated and changes in electrogastrograms (EGG) were analyzed in each subject. Subjects were exposed to a rotating optokinetic drum to induce vection. EGG was recorded before, during, and after the rotation exposure. The severity of nausea which is one of main symptom of motion sickness was suppressed and the percentage of normal band of EGG activity was increased in the retaining needle group than in no treatment group. From these results, it was considered that the retaining needle stimulation of the PC6 acupoints was effective for optokinetic motion sickness possibly by suppressing abnormal gastric myoelectric activities.