New metastasis regulated genes from human lung carcinoma to functional analysis and application for therapy

原发肿瘤的转移是通过许多过程得出的结论。对肿瘤的治疗方法进行分析，是关系到患者预后的重要问题。我们建立了只向肺转移的肺癌细胞系。在转移细胞和非转移细胞之间进行减法。我们分析了与转移有关的四个基因。层粘连蛋白受体（Laminin receptor， LR）基因是细胞粘附层粘连蛋白的特异性受体。通过分析，证明了该基因中编码67kDa分子的部分缺失了外显子。PAF- ah是血小板产生的磷脂活化因子（PAF）的乙酰化酶，与肿瘤转移有关。它与神经细胞的运动有关，但癌症的转移尚不清楚。由于PAF- ah基因在非转移细胞系中的表达，提示PAF失活参与转移控制。在此基础上，采用RACE方法对两个未知基因片段进行分析，得到了该扩展的基因。这些基因均与黑色素瘤有关，其中1个存在于14条染色体上，与子宫颈癌和肠粘膜蛋白有关，最近已被基因库录入与之一致，推测这些基因可能是与自然转移机制有关的分子，但尚未得到转移的功能事件。

A metastasis from primary tumor is concluded through many processes. It is the important problem, which relates to the patient's prognosis closely and to make this analysis of the therapy for the cancer. We established the lung cancer cell line, which it only metastasis to the lung. Subtraction was carried out between metastasis and non-metastasis cells. We analyzed four genes, which related to the metastasis. Laminin receptor (LR) gene is specific receptor toward cell adhesion protein to laminins. It was proved that there was a loss of exson in a part of the gene that a code does a 67kDa molecule as an analysis. And, PAF-AH is acetylhydlase of the activated factor of the phospholipids (PAF), which produced of the platelets, which relates to the tumor metastasis. It is involved in the movement of the nerve cell, but the cancer metastasis isn't clear. Participation is suggested to the metastasis control due to inactivation of PAF due to the expression of this PAF-AH gene with the non-metastasis line. Furthermore, RACE method was enforced, and it got the gene of the extension to analyze two unknown genetic fragments. These genes are related to the melanoma, one of others existed in 14 chromosome, which relates to the uterine cervix carcinoma and the intestine mucous membrane protein, and agreement were entry of Gene bank with it recently The genes are presumed probably to be the molecule, which relates to the natural metastasis mechanism but couldn't get the functional events for metastasis.