An investigation of underlying mechanism of delayed inflammatory state and hyperalgesia located in a healed wound-A study of involvement of sympathetic activity and possibility of therapeutical effect of preemptive sympathectomy

1、先发性交感神经切除可防止后爪炎性改变（肿胀、发红）的发生，但不影响去甲肾上腺素对慢性炎症大鼠c纤维伤害感受器的增强作用。这表明慢性炎症状态下炎症过程的发展和伤害感受器的肾上腺素能敏感性之间的潜在机制存在根本差异。2、神经病变大鼠血浆去甲肾上腺素有明显的日变化。3、热刺激后，低浓度的ATP（模拟交感递质剂量）激活了部分cpr，但随后的热反应减弱。较高浓度的ATP（模拟损伤细胞的剂量）激发了一些cpr，增加了热反应。在crps中，ATP似乎在交感维持的痛觉过敏中不占主导地位。4 .慢性炎症诱导的皮肤c纤维伤害感受器的去甲肾上腺素敏感性在Lewis品系和Sprague-Dawley组之间存在明显差异。这表明，交感神经切除术治疗效果的广泛范围是由于每个受试者的交感神经依赖性不同。

1, Preemptive sympathectomy prevented the development of inflammatory changes (swelling, redness) in the hindpaw, while but not influence the augmenting effects of noradrenaline on the C-fiber nociceptors of chronic inflamed rats. This suggests fundamental differences of underlying mechanism between developments of inflammatory process and adrenergic sensitivity of nociceptors under chronic inflamed condition.2, There was a clear development of daily change in plasma noradrenaline in rats rendered neuropathic. This suggests that sympathetic activity is not stable through the whole period of CRPS.3, Low concentration of ATP (a mimic dose of sympathetic transmitter) excited some of the CPRs after heat stimulations, but the subsequent heat responses were decreased. Higher concentration of ATP (a mimic dose of damaged cell) excited some CPRs and increased the heat responses. ATP seems not to have a predominant role in the sympathetically maintained hyperalgesia in CRPS.4, There were clear differences between the Lewis strain and Sprague-Dawley rate in chronic inflammation induced noradrenaline sensitivity of cutaneous C-fiber nociceptors. This suggests that wide rage of therapeutic effect of sympathectomy is due to difference of sympathetic dependency of each subject.