Tissue-engineered cartilage using cultured chondrocytes and reinforced with a periosteal explant for repair of articular cartilage

组织工程软骨在关节软骨损伤治疗中的应用一直受到人们的关注。然而，其临床应用一直受到其与软骨受损区域结合困难和与下底骨不充分结合的挑战。为了克服这些缺点，我们使用组织工程软骨和骨膜外植体进行了复合移植，进行了组织学和生化评估，并展示了初步的体内数据。将小猎犬关节软骨培养的软骨细胞植入聚乙醇酸无纺布网中，并从同一只小猎犬身上取出新鲜的骨膜外植体缝合在一侧。在培养皿中培养的细胞三维培养导致了透明软骨的构建，其一侧附着在骨膜外植体上。在用骨膜移植体加固工程软骨的连接处观察到骨膜移植体的软骨形成。将自体移植物植入髋臼再生关节软骨全层缺损。由透明软骨和骨膜外植体组成的双层复合组织结构为关节软骨修复提供了一种简单有效的骨软骨移植。

The use of tissue-engineered cartilage in the treatment of articular cartilage damage has been receiving attention. However, its clinical application has been challenged by the difficulty in its binding to the damaged area of the cartilage and its insufficient union with the underlying bone. As a means to overcome the drawbacks, we created a composite graft using tissue-engineered cartilage reinforced with a periosteal explant, conducted histological and biochemical evaluations, and showed initial in vivo data. The cultured chondrocytes derived from an articular cartilage of beagle were seeded onto a polyglycolic acid non-woven mesh with a fresh periosteal explant removed from the same beagle sutured to one side. Three-dimensional culture of the cells grown in petri dishes led to the construction of a hyaline cartilage with one side attached to a periosteal explant. Chondrogenesis of the periosteal explant was observed at the junction where the engineered cartilage was reinforced with a periosteal explant. The autologously grafted construct into a full-thickness defect on the acetabulum regenerated articular cartilage. The two-layered composite tissue construct consisting of a hyaline cartilage and a periosteal explant could provide a simple and effective osteochondral graft for articular cartilage repair.