Correlation between Histological Degree of Chondrosarcoma and Detection of MMps, ADAMTSs and TIPs

背景：仅凭组织学结果很难判断人软骨肉瘤的恶性程度。因此，我们检测了基质金属蛋白酶(MMPs)、去整合素和金属蛋白酶(ADAM)及其抑制物(TIMPs)在软骨肉瘤中的表达，并探讨了它们与软骨肉瘤恶性程度和预后的关系。材料和方法：对28例软骨肉瘤患者分别采用免疫组织化学方法检测基质金属蛋白酶-2、3、7、9、13、ADAMTS-4、ADAMTS-4和TIMP-1、2、3的表达。所有目的蛋白均在大肠杆菌中得到了表达。软骨肉瘤。除MMP9外，MMP2、MMP3、MMP13、ADAMTs和所有3种TIMP的表达与组织学分级均呈正相关(p&lt；0.05)。结论：这些蛋白可提示软骨肉瘤的恶性程度。

Background: Malignant degree of human chondrosarcoma can be difficult to determine using only histological findings. We therefore assessed expressions of matrix metalloproteinases (MMPs), a disintegrin and metalloproteinase (ADAM) with thrombospondin motifs (ADAMTSs) and tissue inhibitor of metal I oprotei nases (TIMPs) in chondrosarcoma and ascertained relationships to histological degree of malignancy and prognosis.Materials and Methods: In 28 chondrosarcoma patients, inimunostaining was performed using antibodies for each of the proteins of MMP-2, 3, 7, 9, 13, ADAMTS-4, 5 and TIMP-1, 2, 3.Results: Chondrosarcoma were classified into 7, 15 and 6 cases in histologically malignant grade I, 11 and 111, respectively. All target proteins were expressed in. chondrosarcoma. Positive correlations (p<0.05) existed between immunostaining scores and histological grades for all proteins except MMP 9, with strong correlations (p<0.01) for MMPs 2, 3 and 13, both ADAMTSs and all 3 TIMPs. No correlation existed between prognosis and immunostaining scores.Conclusions: These proteins could thus indicate degree of malignancy in human chondrosarcoma.