Identification of genes associated with relapse of prostate cancer and gene therapy

我们将LNCaP细胞（雄激素敏感的人前列腺癌细胞）接种于5周龄的scid小鼠皮下，并添加50%的基质。目视肿瘤增大后，将小鼠阉割。首先，肿瘤发育迟缓2周。然后它说要放大。我们将小鼠处死，取肿瘤进行传代培养。(LN-RECO)。接下来，我们将LN-RECO接种于阉割小鼠。再次取肿大的肿瘤进行传代培养（LN-REC4）。我们对LN-REC4细胞进行了表征。尽管体外雄激素刺激LN-REC4细胞，但其在去势小鼠体内的生长速度要快于LNCaP细胞，提示LN-REC4可能是反映前列腺癌复发的良好模型。我们还研究了COX-2基因，因为该基因似乎与前列腺癌的进展有关。LNCaP细胞过表达COX-2基因可诱导细胞体外和体内增殖，诱导VEGF分泌。

We inoculated LNCaP cells, androgen-sensitive human prostate cancer cells, onto 5-week aged scid mice subcutneously with 50 % matrigel. After tumor was increased visually, we castrated the mouse. First, tumor retarded for 2 weeks. Then it stated to enlarge. We sacrificed the mouse and took the tumor and subcultured. (LN-RECO). Next we inoculated the LN-RECO to the castrated mouse. Again we took the enlarged tumor and subcultured (LN-REC4).We characterized LN-REC4 cells. Although LN-REC4 cells were stimulated by androgen in vitro, the growth speed of those cells in the castrated mouse was faster than LNCaP cells, suggesting that LN-REC4 seemed to be a good model that reflect the relapse of prostate cancer.We also investigated the COX-2 gene since this gene seemed to be related with progression of prostate cancer. Overexpression of COX-2 gene in LNCaP cells induced cell proliferation in vitro and in vivo, and induced VEGF secretion.