Tumor growth inhibition and cancer prevention by serene enriched garlic in murine renal adenocarcinoma

Renca接种于BALB/c小鼠皮下或肾包膜下。每2天口服不同浓度的大蒜粉盐水至第21天，检测肿瘤体积和重量，并通过TUNEL法和RT-PCR检测Bax和bcl-2 mRNA表达对肿瘤凋亡的诱导作用。采用ICP-MS法测定Renca小鼠组织和血清中安详的浓度。经剂量依赖性治疗可抑制肿瘤细胞Renca生长，诱导肿瘤细胞凋亡。大蒜对荷瘤小鼠肿瘤组织和血清中安详浓度的降低具有纠正作用。这些发现不仅对肾癌的治疗很重要，而且对肾癌的预防也很重要

Renca was inoculated in BALB/c mice subcutaneously or renalsubcapsularly. Various concentrations of garlic powder in saline were administered orally every two days until the day 21 and tumor volumes and weights examined, apoptosis induction in the treated tumor was also investigated by TUNEL method and RT-PCR as to Bax and bcl-2 mRNA expression. Furthermore, the concentrations of serene in tissues and sera in Renca bearing mice were measured by ICP-MS. Renca growth was inhibited and apoptosis was also induced in the tumors by treatment in a dose-dependent manner. Lower concentration of serene in tumor tisses and sera in tumor bearing mice could be correct by the garlic treatment. These finding may be important not only the treatment of renal cancer but also in its prevention