Study on the regulatory mechanism of differentiation and invasive ability of human endometrial stromal cells

子宫内膜是一种特殊的组织，在短时间的月经周期内反复增殖和脱落，对胚胎着床和妊娠的维持起着重要作用。在正常子宫中，子宫内膜间质细胞向宫腔增殖，形成子宫内膜增殖期的功能层。在子宫内膜异位症中，与正常组织相邻的细胞是基质细胞，免疫细胞也存在于它们周围。因此，研究基质细胞的侵袭对于研究子宫内膜异位症的发生发展机制具有重要意义。本研究在知情同意的情况下，从子宫内膜组织中分离子宫内膜间质细胞，利用子宫内膜间质细胞侵袭实验模型，分析其侵袭机制及其调控因素。我们发现子宫内膜间质细胞具有体外侵袭能力。加入类固醇激素、达那唑、蛋白酶抑制剂、细胞因子、非甾体类抗炎药和抗整合素抗体等对基质细胞的侵袭率无明显影响，但与人外周血单个核细胞（PBMC）共同培养时，基质细胞的侵袭率明显增加。在PBMC共培养模型中加入这些因素后，未观察到明显的影响。这些结果表明，正常子宫内膜间质细胞具有侵袭潜能，并且这些侵袭受到免疫细胞的影响，提示这些细胞参与了子宫内膜异位症的发生、发展

Endometrium is a special tissue that repeat proliferation and shedding in a short period of menstrual cycle and play a important role in embryo implantation and the maintenance of pregnancy. In normal uterus, endometrial stromal cells proliferate toward the uterine cavity and form a functional larer of proliferative phase of endometrium. In endometriosis, cells adjacent to normal tissue are stromal cells and immune cells also exist around them. So it should be important to investigate the invasion of stromal cells to study the progressive mechanism of endometriosis. In this study, we isolated endometrial sfromal cells from the endometrial tissue under informed consent and analyzed the invasive mechanism and its regulatory factors using the invasion assay model of endometrial stromal cells. We found that endometrial stromal cells have invasive ability in vitro. Invasion rate of stromal cells was not apparently changed with addition of agents such as steriod hormones, danazol, protease inhibitors, cytokines, NSAIDs and anti integrin antibody, however, the invasion was increased when cells were cultured with human peripheral blood mononuclear cells (PBMC). No apparent effects of those factors were observed when they were added in PBMC co-culture model. These findings demonstrate that normal endometrial stromal cells have invasive potential and those invasion were affected by immune cells, suggested to be involved in progress of endometriosis