A study regarding the mechanism in development of endolymphatic hydrops in Meniere's disease

到目前为止，内淋巴水肿发生的机制尚未阐明。有报道称内耳膜迷路中抗利尿激素v -2受体和水通道AQP-2参与了内淋巴水肿的发生。先前的报道证实加压素给药豚鼠腹腔后在形态学上出现内淋巴积液。然而，该动物模型能否反映包括梅尼埃病在内的内淋巴水肿疾病，尚有待证实。研究1通过腹腔给药加压素，观察内淋巴水肿动物模型（Wistar大鼠）是否发生听力障碍。如Study1的结果证实，71.4%的人出现听力障碍。研究2观察腹腔加压素对内淋巴水肿动物模型听力障碍的时间变化。根据Study2的结果，证实如下：1)加压素治疗后30 ~ 120分钟出现听力障碍，2)听力恢复率为50%，3)听力障碍主要发生在一侧（80%）。在研究3中，通过腹腔给药加压素，研究脱水负荷对内淋巴水肿动物模型听力障碍的影响。如Study3的结果，在脱水24h的内淋巴水肿动物模型中，给药后仅10分钟内出现一侧听力障碍。认为AQP-2是在脱水负荷作用下在细胞内形成的，在加压素的作用下，AQP-2向细胞膜移动，水从毛细血管进入内淋巴腔，发生内淋巴积液。提示脱水负荷使膜迷路细胞为内淋巴积水做好了准备。少

Until now, the mechanism in development of endolymphatic hydrops was not elucidated. There were some reports that V-2receptor for Vasopressin and water channel aquaporin-2 (AQP-2) in inner ear membranous labyrinth participated in development of endolymphatic hydrops. It was confirmed that endolymphatic hydrops was developed morphologically in administering vasopressin to abdominal cavity in guinea pig in previous reports. It was not, however, confirmed that this animal model could reflect the diseases with endolymphatic hydrops including Meniere's disease.In Study1, it was investigated whether the hearing disturbance was occurred in the endolymphatic hydrops animal model (Wistar rat) by administering vasopressin to abdominal cavity. As the results in Study1, it was confirmed that the hearing disturbance was occurred in 71.4%.In Study2, the time course of the hearing disturbance in the endolymphatic hydrops animal model by administering vasopressin to abdominal cavity was investigated. … More As the results in Study2, it was confirmed as follows; 1) the hearing disturbance was occurred in 30-120 minutes after administering vasopressin, 2) the recovery in hearing was found in 50%, 3) the hearing disturbance was mainly in only one side (80%).In Study3, the influence of dehydration load to the hearing disturbance in the endolymphatic hydrops animal model by administering vasopressin to abdominal cavity was investigated. As the results in Study3, in the endolymphatic hydrops animal model that dehydration for 24hrs was loaded, the hearing disturbance in only one side was occurred withn only 10 minutes after administering vasopressin. It was thought that AQP-2 was composed in the cell by dehydration load, AQP-2 moved to the cell membrane by the dosage of Vasopressin, water moved to endolymphatic cavity from the blood capillary and then the endolymphatic hydrops was occurred. It was suggested that dehydration load made the cells in membranous labyrinth get ready for the endolymphatic hydrops. Less