Development of a new sterilization method using ozonated water for the infected carious dentin, pulp, and root canal

臭氧对细菌、真菌、原生动物和病毒有很强的抗菌作用。本课题通过实验研究了臭氧水对感染根管的消毒效果，结果表明，臭氧水（0.5mg/L）作用10-30 s后，感染根管中的优势菌粪肠球菌（Enterococcus faecalis）的活力明显下降。用臭氧水（4 mg/L）处理牛牙感染根管120 s后，粪肠球菌的活菌数比蒸馏水处理的粪肠球菌活菌数减少了10个，几乎未检出细菌。臭氧水与聚维酮碘、苄索氯铵等口腔消毒剂的抗菌活性和细胞毒性无显著差异。臭氧水（4 mg/L）处理120 s后，对混合感染模型的感染根管有很高的抑菌效果，即使在牙本质小管深处也有很好的抑菌效果，提示臭氧水可用于感染根管的消毒。

Ozone is a strong antimicrobial agent against bacteria, fungi, protozoa and viruses. In this project, we examined the sterilization ability of ozonated water on a experimental model for the infected root canal.After exposure to ozonated water (0.5 mg/L) for 10-30 s, the viability of Enterococcus faecalis, one of the predominant bacteria in a infected root canal, significantly decreased. With ozonated water treatment on a experimental model for the infected root canal using bovine tooth, the number of living Enterococcus faecalis was decreased to one tens compared to that with distilled water, and almost no bacteria were detected after being treated with ozonated water (4 mg/L) for 120 s. There were no significant differences in antimicrobial activity and cytotoxicity to human cell line between ozonated water and oral disinfectants such as povidone iodine and benzethonium chloride. After ozonated water (4 mg/L) treatment for 120 s, antimicrobial effect against mixed infection model for the infected root canal was very high level, even in a deep area of the dentinal tubules.Taken together, these results suggest that ozonated water should be useful in the sterilization for infected root canal.