Development of a new sterilization method using ozonated water for the infected carious dentin, pulp, and root canal

臭氧是一种对细菌、真菌、原生动物和病毒具有很强抗菌力的物质。在本课题中，我们在一个感染根管的实验模型上检测了臭氧水的杀菌能力。在感染的根管中，臭氧水(0.5 mg/L)作用10-30个S后，感染根管中的优势菌之一粪肠球菌的活力显著下降。在牛牙感染根管的实验模型上，用臭氧水处理后，与蒸馏水相比，粪肠球菌活菌数减少了10个数量级，经4 mg/L的臭化水处理120个S后，几乎没有检测到细菌，与口服消毒剂聚维酮碘、苯乙氯铵对人细胞株的抗菌活性和细胞毒性无显著差异。经臭氧水(4 mg/L)处理后，对S感染根管的混合感染模型有很高的抗菌效果，甚至在牙本质小管的深部也有很高的抗菌效果，提示臭氧水可用于感染根管的消毒。

Ozone is a strong antimicrobial agent against bacteria, fungi, protozoa and viruses. In this project, we examined the sterilization ability of ozonated water on a experimental model for the infected root canal.After exposure to ozonated water (0.5 mg/L) for 10-30 s, the viability of Enterococcus faecalis, one of the predominant bacteria in a infected root canal, significantly decreased. With ozonated water treatment on a experimental model for the infected root canal using bovine tooth, the number of living Enterococcus faecalis was decreased to one tens compared to that with distilled water, and almost no bacteria were detected after being treated with ozonated water (4 mg/L) for 120 s. There were no significant differences in antimicrobial activity and cytotoxicity to human cell line between ozonated water and oral disinfectants such as povidone iodine and benzethonium chloride. After ozonated water (4 mg/L) treatment for 120 s, antimicrobial effect against mixed infection model for the infected root canal was very high level, even in a deep area of the dentinal tubules.Taken together, these results suggest that ozonated water should be useful in the sterilization for infected root canal.