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Pulp Irritation by Restorative Materials-Influence on Pulp Potential of Dentin Bridge Formation-

修复材料对牙髓的刺激-对牙本质桥形成牙髓潜力的影响-

基本信息

批准号：
06404069
负责人：
TERASHITA Masamichi
金额：
$ 5.5万
依托单位：
Kyushu Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06404069/
关键词：
Restorative materials Pulp irritation Function of pulp cell Dentinogenesis Dentin bridge c-jun jun-B Rat デンティン・ブリッジウシ歯胚遺伝子

项目摘要

This study tested the hypothesis that in the case of restoration tooth, the pulp tissue may react to restoration materials and lead to various dentin bridge formation. The effects of dental materials on the function of pulp cells during teparative dentinogenesis were also rested by the examination of expressions of c-jun and jun-B proto-oncogenes, the essential genes for cell-differential.Maxillary first molars of wister SPF rats were used for histological examination in this study. As the first irritation on pulp cells, class V restoration (eugenol cement, composite resin, amalgam, glassionomer cement, silicate cement) were performed at the mesial site of the first molars. Two weeks later, calcium hydroxide pulpotomy at the occlusal site of the same tooth was performed as the second irritation. The pulp irritation from the class V restoration materials were examined by measuring the thickness of reparative dentin bridge formed at the plupotomy site for four weeks after pulpotomy. Teet … More h without class V restoration were used as control groups. Compared to the control group, less dentin bridge formation at the composite resin filing group were found. Especially at the first week, it showed lessdentin bridge formation than other groups. In both of the glassionomer cement and silicate cement groups, the polyacrylic acid and phosphoric acid were used as liquid, a significantly increasing in dentin bridge formation were observed, especially during the second week interval. In the case of amalgam filling group, a constantly increasing in dentin bridge formation was found. Eugenol cement filling groups showed a similarly values of dentin bridge formation with the control group through the whole experiment interval. Further more, the expressions of c-jun and jun-B were found only in the odontoblastic lineage both of bovine and rat tooth germs, and temporal and spatial expressions of c-jun and jun-B in cells associated with reparative dentinogenesis after cavity preparation.It can be concluded, beside the conventional morphological evaluation of biocompatibity of dental materials, it is necessary to examine the function of the pulp tissue. This results also suggest that c-jun and jun-B may play important roles in the cell associated with primary and reparative dentinogenesis and these genes can become molecular markers of pulp cells to assess the effects of dental materials on pulp cell. Less

本研究验证了在修复牙的情况下，牙髓组织可能会对修复材料产生反应，导致各种牙本质桥的形成。通过检测细胞分化的关键基因c-jun和jun-B原癌基因的表达，探讨牙材料对分离牙本质形成过程中牙髓细胞功能的影响。本研究采用wister SPF大鼠上颌第一磨牙进行组织学检查。作为对牙髓细胞的第一次刺激，在第一磨牙的内侧位置进行V类修复（丁香酚水泥、复合树脂、汞齐合金、玻璃聚合物水泥、硅酸盐水泥）。2周后，在同一牙的咬合部位行氢氧化钙切髓术，作为第二次刺激。在截髓后4周，通过测量在截髓部位形成的修复牙本质桥的厚度来检测V类修复材料对牙髓的刺激。未进行V级修复的小鼠作为对照组。与对照组相比，复合树脂锉组牙本质桥的形成较少。特别是在第一周，牙本质桥的形成比其他组少。在玻璃水门汀组和硅酸盐水门汀组中，聚丙烯酸和磷酸作为液体使用时，牙本质桥的形成明显增加，特别是在第二周期间。在汞合金充填组，牙本质桥的形成不断增加。丁香酚水泥充填组在整个实验周期内牙本质桥的形成值与对照组相近。此外，c-jun和jun-B仅在牛和大鼠牙胚成牙系中表达，并且c-jun和jun-B在造腔后修复性牙本质发生相关细胞中的时空表达。由此可见，除了常规的牙材料生物相容性形态学评价外，还需要对牙髓组织的功能进行检查。c-jun和jun-B基因可能在牙本质发生和修复过程中发挥重要作用，可以作为牙髓细胞的分子标记来评价牙髓材料对牙髓细胞的影响。少