Study of Denture Plaque Adhesion to Denture Surfaces with Different Surface Conditions

用激光显微镜观察树脂黏附念珠菌在义齿表面的粘附情况。采用激光共聚焦显微镜观察了念珠菌黏附义齿表面的分布情况。水溶性伊红浓度为0.01%，染色时间为30秒，冲洗时间为60分钟。这种方法优于现有的方法，因为它在低倍率下提供了假丝酵母分布的清晰图像。它可以用来准确地评估整体念珠菌粘附在义齿表面。不同表面条件下念珠菌对义齿表面粘附的研究-原子力显微镜观察义齿表面条件-观察临床常用义齿衬中念珠菌的粘附情况。衬里条件为400、1000、5μ。荧光观察采用激光显微镜。CFU单位用于表示念珠菌粘附程度。表面用原子力显微镜观察。所有病例的依从性都很好。然而，原子力显微镜检查显示，念珠菌在粗糙表面的粘附比在光滑表面的粘附更大。原子力显微镜提供了更高的清晰度和精度。

Study of Microorganism Adherence to Denture Surfaces Using a Laser Microscopic- Observation of Resin Adhesion Candida at Low Magnification -A confocal laser microscopic was used in a simple procedure to observe the distribution of Candida adhering denture surfaces. Water soluble eosin (0.01% density with a dyeing time of 30 seconds and a flushing time of 60 minutes) was found to be appropriate for this method.This method is superior to existing methods because it provides a clear picture of Candida distribution at low magnification. It can be used to accurately evaluate overall Candida adherence to denture surfaces.Study of Candida Adhesion to Denture Surfaces with Different Surface Conditions-Atomic Force Microscope Observation of Denture Surface Conditions -Candida adhesion to denture linings commonly used in clinical applications was observed. Lining conditions varied "400, "1, 000, 5μ.Fluorescent observation used a laser microscopic. CFU units were used to represent degrees of Candida adherence. Surface observations were made with atomic force microscope.Adherence was substantial in all cases. However, atomic force microscope examination showed greater Candida adherence on the rough surfaces than on the smooth surfaces.The Atomic force microscope provides greater clarity and precision.