The effects of particulate metals on osteoblast-like cells in vitro

在体外研究了15种金属微粒对成骨细胞样细胞活力的影响，以确定颗粒形式或可溶性离子是否会对细胞产生不利影响。此外，通过MTT和碱性磷酸酶活性分析，研究了6种非腐蚀性颗粒金属对两种成骨样细胞的剂量效应。Al、Ti、Zr、Nb、Ta、Cr、Mo和Fe颗粒在较高浓度下会抑制细胞活力，但除Mo外，它们的提取物对细胞没有影响。另一方面，Cu、Si、V、W和Co颗粒与提取物对细胞活力的影响差异不大，而Mn和Ni颗粒对细胞的不利影响大于它们的提取物。此外，MG-63与非腐蚀性颗粒金属的相互作用导致MG-63和HOS细胞之间的细胞毒性结果不同。MG-63细胞的细胞活力仅在较高浓度下受到抑制，而HOS细胞的细胞活力在低浓度下受到抑制。与Nb、Ta和Cr颗粒相比，Al、Ti和Zr颗粒更明显地抑制细胞活力。较高浓度的Al颗粒增加了ALP活性，而其他颗粒则没有。

The effects of fifteen particulate metals on cell viability of osteoblast-like cells were studied in vitro, to determine whether an adverse effect on cells could be induced by the particulate forms or soluble ions. In addition, the dose-effects of six noncorrosive particulate metals on the two osteoblast-like cells were studied by analyzing MTT and alkaline phosphatase activity. The Al, Ti, Zr, Nb, Ta, Cr, Mo, and Fe particulates depressed cell viability at higher concentrations, but their extracts yielded no effect on cells, except for Mo. On the other hand, little difference in cell viability between particulates and extracts was observed for Cu, Si, V, W, and Co, while Mn and Ni particulates yielded more adverse effects on cells than did their extracts. Moreover, the interaction with noncorrosive particulate metals led to different cytotoxic results between MG-63 and HOS cells. The cell viability of MG-63 cells was depressed only at higher concentrations, but that of HOS cells was depressed at low concentration. Al, Ti, and Zr particulates more markedly depressed cell viability compared with Nb, Ta, and Cr particulates. Al particulates at higher concentrations increased ALP activity, but other particulates did not.