Feasibility study for an evaluation system of effect and side effect chemotherapy for oral cancer utilizing time PCR

本课题的目的是利用真实的时间PCR技术建立一个较为准确的口腔癌化疗疗效和副作用的评价体系。为此，我们试图检测口腔鳞状细胞癌患者化疗过程中细针穿刺获得的外周血细胞和肿瘤细胞中凋亡相关蛋白的mRNA表达。然而.由于尚未建立此类检查，因此开始了使用经或未经抗癌剂处理的培养细胞的可行性研究。经顺铂和/或5-FU治疗后，部分患者外周血细胞凋亡诱导蛋白Fas、Bax、Caspase-2、3、8、9的mRNA表达增加。另一方面，经顺铂和（或）5-FU处理后，部分病例的外周血细胞中Bcl-2、Bcl-X_1、c-IAP 2、XIAP等凋亡抑制蛋白的mRNA表达增加。这些结果表明，外周血细胞可能是敏感的抗癌药物在化疗过程中，和抗癌药物的副作用，可以评估通过检查的凋亡相关蛋白的mRNA表达。目前，我们尝试将口腔癌患者的外周血细胞用于口腔癌化疗的临床研究。在本研究中，我们将研究糖尿病相关蛋白的mRNA表达水平与副作用的临床表现之间的关系。由于通过使用外周血细胞的可行性研究建立的技术诀窍适用于使用细针穿刺获得的肿瘤细胞的检查，因此本项目中提出的评估系统将产生有关抗癌剂效果的有用信息。

The aim of this project is establishment of a mere accurate evaluation system of effect and side effect of chemotherapy for oral cancer utilizing real time PCR. For this purpose, we attempted to examine the mRNA expression of apoptosisrelated proteins in peripheral blood cells and tumor cells of patients with oral squamous cell carcinoma obtained by fine needle aspiration during the course of anticancer chemotherapy. However. Since this kind of examination has not been established so started on a feasibility study using culture cells treated with or without anticancer agents. After the treatment wife cisplatin and/or 5-FU, mRNA expression of apoptosis-mediating proteins inducing Fas,Bax, Caspase-2,3,8,9 in the cultured peripheral blood cells was increased in some cases. On the other hand, in the cultured peripheral blood cells after the treatment with cisplatin and/or 5-FU, some cases also showed increased expression of mRNA of apoptosis-suppressing proteins incliding Bcl-2, Bcl-X_1, c-IAP2, XIAP. These findings suggest that peripheral blood cells may be susceptible to anticancer agents during the course of chemotherapy, and the side effect of the anticancer agents can be evaluated be evaluated by examination of mRNA expression of apoptosis-related proteins. At preaent, we anttempt to start on the clinical study using peripheral blood cells of patients with oral cancer in the course of anticancer chemotherapy. In this study, we will examine the relationship between the expression level of mRNA of apoptosis-related proteins and clinical manifestation of the side effects. Since the know-how that is estabished by feasibility study using peripheral blood cells is applicable to the examination using tumor cells obtained by fine needle aspiration, an evaluation system proposed in this project will yield useful information conceming the effect of anticancer agents.