Quantity and polymorphism analysis of fMLP receptor gene in patients with prepubertal periodontitis

N-甲酰-甲硫氨酰-亮氨酰-苯丙氨酸（fMLP）肽是通过与fMLP受体结合而起作用的吞噬细胞化学引诱剂。本研究采用真实的时间RT-PCR和直接测序技术对8例青春期前牙周炎患者及其8名直系亲属的fMLP受体mRNA进行了系统的分析。对于fMLP受体I，在所有患者（6.7-83.7%）和4名家族成员（17.5-84.2%）中检测到抑制的趋化活性。趋化活性降低时，fMLP受体mRNA表达有增加（1.18-8.37倍）和减少（0.05-0.08倍）两种模式。如果一个孩子有抑制的趋化活性，那么父母中至少有一个也被发现有抑制的活性。对于fMLP受体I，本研究中未检测到SNPs，但通过真实的RT-PCR分析，在青春期前牙周炎患者及其直系亲属的fMLP受体基因上可能存在SNPs位点。

N-formyl-methionyl-leucyl-phenylalanine (fMLP) peptide are phagocyte chemoattractants that act by binding to fMLP receptor. Here I systematically analyzed the amount of fMLP receptor mRNA by real time RT-PCR and direct sequence of cloned alleles from blood donors from the 8 patients with prepubertal periodontitis and 8 immediate family members. For fMLP receptor I detected depressed chemotactic activity in all present patients (6.7-83.7%) and four family members (17.5-84.2%). There were two patterns found of the increased (1.18-8.37 times more than that of the control) and decreased (0.05-0.08 times less than that) fMLP receptor mRNA in the depressed chemotactic activity. If a child has the depressed chemotactic activity, then at least one of the parents was also found of the depressed activity. For fMLP receptor I detected no SNPs in a sample of the present study, however, there could be some sites of SNPs in fMLP receptor gene of the prepubertal periodontitis patients and immediate family members by real time RT-PCR analysis.