BIOREMEDIATION OF SOIL CONTAMINATED BY OIL AND DIOXINS

采用矿物加工技术和生物修复技术对石油和多氯化合物污染土壤进行了净化研究。在日本Amaraman油井油水分离器水相中抑制的细菌种类主要有假单胞菌、γ-变形菌和希瓦氏菌。将含菌水直接或培养后加入含油土壤或含二恶英土壤中进行生物降解试验，对含油约3%的老加油站实际污染土壤进行气浮处理，除油率达60%。然后，将细菌溶液加入土壤中并通过塔磨机搅拌。两周后，50%的油可以从土壤中去除。5周后，细菌对土壤中总石油烃的去除率为70%。浮选产品中二恶英的浓度是浮选产品的10倍。另一方面，研究了人工二恶英污染土壤的生物修复。培养菌对污泥（75%的水）中合成的PCDD/Fs的去除率在4周内超过80%。在土壤中（33%的水），细菌对PCDD/Fs的去除率在四周内约为35%。油井抑菌对土壤中的石油和二恶英有很好的降解作用。

To purify the oil and porychlorinated compound contaminated soil was studied with mineral processing techniques and bioremediation. Bacteria species that inhibit in aqueous phase in the separator of water from crude oil in Amarume oil well in Japan are mainly pseudomonas species, gammaproteobacteria classis and shewanella species. This water including bacteria is added directly or after cultivation, mixed with the soil containing oil or dioxins and then the biodegradations were measured.The actual oil contaminated soil including about 3% oil from old gas station was treated by the flotation and the oil removal rate was 60%. Next, the bacteria solution is added in the soil and agitated by tower mill. After 2 weeks the 50% of oil could be removed from the soil. The removal of total petroleum hydrocarbon in soil by bacteria is 70% after 5 weeks.The actual soil with incinerated ash containing dioxins was treated by flotation. The dioxins were concentrated 10times in the float products. On the other hand, the bioremediation of soil containing artificial dioxins was investigated. The removal of synthesized PCDD/Fs in mud (75% water) by cultivated bacteria is more than 80% decomposition rate over four weeks. The removal of synthesized PCDD/Fs in soil (33% water) by bacteria is around 35% decomposition rate in four weeks. The bacteria inhibiting in crude oil well is very effective to decompose oil and dioxins in soils.