Basic study on cartilage, bone and tendon tissue engineering by using physical factors

维持生活质量已成为社会关注的问题。为了通过制造组织工程化骨、软骨和肌腱来维持QOL，我们试图利用这些组织的特性[即，它们对诸如机械应力、磁刺激等物理因素敏感]。首先，对细胞增殖和分化条件进行了优化。为了制备不含胎牛血清（FBS）的间充质细胞增殖培养基，并在大约一个月内提供超过1000倍的细胞数量增加，我们通过部分析因设计中的方差分析研究了12个因素对人细胞增殖的组合效应。结果，FGF-2、地塞米松、胰岛素和IGF-I对软骨细胞的增殖具有促进作用，而FGF-2与胰岛素或IGF-I的组合协同增强增殖。因此，我们得出结论，这种组合的FGF-2与胰岛素或IGF-I可能是有用的促进耳廓软骨细胞增殖的软骨再生的临床应用。接下来，我们发现BMP-2、胰岛素和甲状腺激素（BIT）的组合适合于解决沿着长时间细胞培养引起的软骨细胞去分化的问题。然后，我们通过研究它们对细胞增殖或分化的影响，研究了一些有望用于临床应用的水凝胶的特性。(228字）

A social concern has risen to the maintenance of quality of life (QOL). Aiming at the maintenance of QOL by making tissue engineered bone, cartilage and tendon, we tried to use the characteristics of such tissues [i.e. ; they sensitively react to physical factors such as a mechanical stress, a magnetic stimulation or etc]. First, the cell proliferation and the differentiation condition were optimized. To prepare a mesenchymal cell proliferation medium that does not contain fetal bovine serum (FBS), and that provides more than a 1000-fold increase in cell numbers within approximately a month, we investigated the combinational effects of 12 factors on the proliferation of human cells by analysis of variance in fractional factorial design. As a result, FGF-2, dexamethasone, insulin, and IGF-I possessed promotional effects on proliferation on chondrocytes, while the combination of FGF-2 with insulin or IGF-I synergistically enhanced the proliferation. Thus we concluded that such a combination of FGF-2 with insulin or IGF-I may be useful for promotion of auricular chondrocyte proliferation in a clinical application for cartilage regeneration. Next we discovered that the combination of BMP-2, insulin, and thyroid hormone (BIT) is suitable for resolving the problem of de-differentiation of the chondrocyte caused along with the long time cell culture. Then, we studied the characteristics of some hydrogels expected to be used in clinical application by investigating their effects on cell proliferation or differentiation. (228 words)