Research on Bio-Photonic Devices Based on Distributed Architecture

整个研究的主题是CMOS图像传感器在生物成像应用中的应用。我们采用了CMOS技术制造的传感器，由于多参数传感的可能性。在这项工作中，我们已经探索和解决了许多问题，相关的光敏，荧光检测，封装芯片成像，和电刺激。当应用于体内成像时，最初我们在小鼠大脑内提供足够的激发光时遇到了一些困难。然而，在体内验证成像期间，通过使用脑体模和来自光纤尖端的照明光解决了该问题。我们发现，当尖端被带到距离传感器表面约1mm时，获得足够均匀的激发光。这最终导致成功的AMC荧光团的体内荧光成像和定量测量。我们相信这项工作是重要的，因为它代表了第一个报告的工作，使用CMOS图像传感器的功能成像内完整的小鼠大脑。从这项工作中，我们已经获得了重要的洞察脉冲调制光敏方法，并意识到，需要更多的工作，以开发一个脉冲调制为基础的图像传感器与快速帧速率成像。

The main theme throughout this entire research is the application of CMOS image sensors for bioimaging applications. We adopted the use of a CMOS technology for fabrication of the sensor due to the possibility of multiparameter sensing. In this work, we have explored and solved many issues related to photosensing, fluorescence detection, packaging for on-chip imaging, and electrical stimulation. When applied for in vivo imaging, initially we encountered some difficulties in providing sufficient excitation light inside the mouse brain. However, this issue was resolved during in vivo verification imaging by using the brain phantom and illumination light from a fiber tip. We found that when the tip is brought to about 1 mm from the sensor surface, sufficiently uniform excitation light was obtained. This has finally led to the successful in vivo fluorescence imaging and quantitative measurement of the AMC fluorophore. We believe that this work is important as it represents the first reported work of using a CMOS image sensor for functional imaging inside the intact mouse brain. From this work, we have gained important insight into the pulse modulation photosensing method and realized that more work would be needed in order to develop a pulse modulation based image sensor with fast frame rate imaging.