Development of a Novel Evaluation Technology of Cell Attachment Ability onto Polymeric Materials Based on Quartz Crystal Microbalance (QCM) Technique

我们已经开发了一种新型的光响应细胞培养表面（POPS），并成功地通过光照射控制了POPS的细胞附着能力。在本研究中，我们旨在利用石英晶体微天平（QCM）技术揭示改变细胞附着特性的机制。基于Sauerbrey定律，假设QCM石英传感器的频率变化与传感器重量的变化成正比，因此QCM可以检测到传感器上极少量的吸附。在2004年的研究中，我们发现我们可以用QCM来评估光照下少量细胞在pps上的附着和脱离。2005年，我们将QCM技术不仅应用于ppps，还应用于几种材料，发现细胞在材料表面的附着主要是细胞与材料之间的物理化学相互作用，这种相互作用发生在细胞接近表面之后。然而，由于pps和细胞是膨胀良好的弹性体，发现基于Sauerbrey定律的传统QCM技术不适用于该系统。然后，我们关注了QCM-D，它可以在石英传感器上检测材料的弹性变化，并通过QCM-D的演示机简要地获得了一些关于细胞表面相互作用的新的有价值的信息。现在，我们正在准备发表这一结果的手稿。顺便说一句，我们用其他预算购买了QCM-D机器，并正在进行进一步的实验。基于这个项目的成果，我们可以更好地设计ppps，我们已经开始了一个新的项目，关于“二维细胞操作系统的开发”，这是日本科学技术振兴机构2005年大学创业创业支持计划的预算

We have already developed a novel photo-responsive cell culture surface (POPS) and been successful to control the cell attachment ability of the POPS by light irradiation. In this study, we were aiming to reveal the mechanism switching the cell attachment property using quartz crystal microbalance (QCM) technique.Based on the Sauerbrey law, the change in the frequency of a quartz sensor of QCM is assumed to be proportional to the change in the weight of the sensor, so that QCM can detect extremely little adsorption onto the sensor. As the result of this study in 2004, we have found that we can evaluate the attachment and detachment of a small number of cells on the PCPS under light irradiation using QCM. In 2005, we applied the QCM technique to not only the PCPS but also several materials and have found that cell attachment on the surface of a material is dominated by physicochemical interaction between the cells and material, which occurs just after the cells approach the surface. However, it was found that the conventional QCM technique based on the Sauerbrey law is not applicable to this system, because the PCPS and cells are well-swelled elastic bodies. Then, we paid attention to QCM-D, which can detect the change in the elasticity of a material on a quartz sensor, and have had several new and valuable information about cell-surface interaction using a demonstration machine of QCM-D briefly. Now, we are preparing a manuscript about this result for publication. Incidentally, we purchased the QCM-D machine with the other budget and are proceeding with further experiments.Based on the results of this project, it has been possible for us to design PCPS much better, and we have started a new project concerning to "Development of Two-Dimensional Cell Manipulation System" with the budget of the Creation and Support Program for Start-ups from Universities in 2005 of Japan Science and Technology Agency