Physiological polymorphisms on sebaceous gland activity in human

将28名18~23岁男性志愿者随机分为3组。第一组(C组)9人在秋季每晚穿全棉内衣，连续4周；第二组(P组)9人在同一时期穿类似涤纶内衣；第三组(AC组)10人穿棉(60%)、涤纶(20%)和双糖(20%)混纺内衣。这3种内衣的吸湿率分别为7.75、0.63和6.46%。我们每周早上用有机溶剂用杯法从他们的背部皮肤收集一次皮脂。色谱图显影后炭化，用岛津CS-9300PS密度计对脂类进行定量。根据峰面积比计算角鲨烯(SQ)、蜡酯(WE)和甘油三酯(TG)的含量。棉质内衣中WE和TG的浓度在实验第3周时逐渐增加，分别为162%和132%，而涤纶内衣中WE和TG的浓度在实验第2周前有一次增加，但在实验结束时分别下降到78%和80%，低于棉质内衣。对纯棉和涤纶内衣进行了混纺实验。3种不同材料的SQ结果无明显变化趋势。因此，与具有亲水性的合成服装相比，具有疏水性能的合成服装似乎在秋季到冬季抑制了自然皮脂腺的活动。结果还表明，合成纤维服装可能会干扰自然的生理变化，与疏水性的棉质服装相比，具有疏水性的涤纶服装会使人体承受更大的压力。然而，尿儿茶酚胺在内衣材料中没有表现出这种趋势。

Twenty-eight male volunteers aged 18-23 years old were divided into 3 groups at random. The first group (C) consisted of 9 persons wore full body cotton underwear every night for 4 weeks in autumn, the second group (P) consisited of 9 persons wore similar polyester underwear for an identical period, and the third group (AC) consisted of 10 persons wore a cotton (60%), polyester (20%) and biscose (20%) blended underwear. The hygroscopicity of these 3 underwear is 7.75, 0.63, and 6.46%, respectively. We collected sebum from their back skin once in the morning every week by a cup method using organic solvents. The chromatograms were developed and then charred, and the lipid classed were quantified by densitometry (Shimazu, CS-9300PS). The amounts of squalene (SQ), wax ester (WE), and triglycerides (TG) were calculated from the ratio of the peak area. The rate of concentrations of WE and TG increased gradually in cotton underwear to 162% and 132%, respectively until the 3rd week of the experiment, while those for polyester underwear increased once until the second week, but later they decreased to 78% and 80%, respectively, at the end of the experiment which was less than in cotton underwear. The result of the blended underwear (AC) was obtained between cotton and polyester underwear. There was no tendency in the result of SQ data among the 3 different kinds of materials. As a result, synthetic clothing with hydrophobic properties seems to suppress natural sebaceous gland activity from autumn to winter compared with that with hydrophilic properties. The results also suggest that synthetic clothing may possibly disturb natural physiological variations and the polyester clothes with hydrophobic properties would make the body more stressful, compared with cotton clothes with hydrophobic ones. However, the urine catecolamines showed no such tendency among the underwear materials.