Employing smart underwear to measure gut microbial hydrogen sulfide production

利用智能内衣测量肠道微生物硫化氢的产生量

• 批准号: 10425072
• 负责人: Reza Ghodssi
• 金额: $ 22.82万
• 依托单位: UNIV OF MARYLAND, COLLEGE PARK
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-10 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10425072
• 关键词: Address; Affect; Anogenital region; Biological Availability; Calories; Clinical Trials; Cohort Studies; Collection; Colorectal Cancer; Complex; Coupled; Cysteine; Devices; Diet; Dietary Factors; Dietary Intervention; Disease; Enzymes; Etiology; Filtration; Flatulence; Future; Gases; Goals; Health; Human; Hydrogen; Hydrogen Sulfide; In Vitro; Intake; Measurement; Measures; Metabolism; Methane; Methods; Miniaturization; Noise; Nutrient; Outcome; Participant; Phase; Physiological; Physiology; Play; Production; Randomized; Research; Role; Signal Transduction; Signaling Molecule; Source; System; Techniques; Testing; Time; Ulcerative Colitis; Validation; arm; comparison intervention; design; dietary; experience; feeding; flexibility; gut microbiota; human microbiota; metal oxide; microbial; microbiome; miniaturized device; precision nutrition; prototype; rectal; sensor; software development; temporal measurement; trimethylamine; wearable device

PROJECT SUMMARY/ABSTRACT The circulating gasotransmitter H₂S is endogenously produced by tightly-regulated human enzymes and exogenously produced in a diet-dependent manner by the human microbiota. Exogenous H2S production by the human gut microbiota influences human health by modulating systemic H2S bioavailability. Excessive gut microbial H2S production is hypothesized to be involved in the etiology of colorectal cancer and Ulcerative Colitis. Our understanding of how gut microbially produced H₂S affects human health remains incomplete because current measurement techniques are imprecise, invasive, require biospecimen collection, and have limited temporal resolution. In this proposal, our objective is to address this gap by developing a research- grade wearable Smart Underwear device that enables long-term real time measurements of gut microbial H₂S production in gaseous rectal effluent (flatus). Flatus are an untapped source for measuring gut microbial H2S production. We have demonstrated that the Smart Underwear v1 prototype can precisely measure physiologically-relevant concentrations of H2S in vitro. When worn by humans, the Smart Underwear v1 prototype detects flatus with an excellent signal-to-noise ratio and is able to estimate the concentration of H2S in each flatus. In the R21 phase, we propose to develop a second revision Smart Underwear prototype for clinical trials through miniaturization, software development, and incorporation of flexible materials. In addition, we propose to conduct a user experience trial to gauge the user acceptance and test the reliability of the Smart Underwear device when worn by humans. In the R33 phase, we will use the Smart Underwear v2 prototype developed in the R21 phase to test the hypothesis that the Smart Underwear device can detect diet-induced alterations in gut microbial H2S production using a randomized controlled feeding trial that contrasts gut microbial H₂S production by modulating dietary cysteine intake. The completion of the proposed goals will lead to the first wearable device that enables long-term real time measurements of gut microbial H2S production. This will facilitate the design of personalized dietary interventions to rationally modulate gut microbial H₂S production and benefit future studies seeking to untangle the complex interactions between gut microbial H2S production and disease.

项目总结/摘要 循环气体传递素H β S由严格调节的人类酶内源性产生， 由人类微生物群以饮食依赖的方式外源产生。外源H2S的产生 人类肠道微生物群通过调节全身H2S生物利用度来影响人类健康。肠道过多 微生物H2S的产生被假设参与结肠直肠癌和溃疡性结肠炎的病因学。 结肠炎。我们对肠道微生物产生的H2S如何影响人类健康的理解仍然不完整 因为当前的测量技术是不精确的、侵入性的、需要生物样本收集，并且 有限的时间分辨率在这项建议中，我们的目标是通过开发一项研究来解决这一差距- 一种可穿戴的智能内衣设备，能够长期真实的时间测量肠道微生物的硫化氢 气体直肠流出物（肠胃气）中的产生。肠胃气是测量肠道微生物H2S的未开发来源 生产我们已经证明，智能内衣v1原型可以精确测量 H2S的体外生理相关浓度。当被人类穿着时，智能内衣v1 原型以出色的信噪比检测肠胃气，并能够估计H2S的浓度 在每一次放屁。在R21阶段，我们建议开发第二版智能内衣原型， 通过微型化、软件开发和柔性材料的结合进行临床试验。此外，本发明还提供了一种方法， 我们建议进行使用者体验试验，以评估使用者的接受程度和测试智能 内衣装置时，穿的人。在R33阶段，我们将使用智能内衣v2原型 在R21阶段开发，以测试智能内衣设备可以检测饮食诱导的假设 使用随机对照喂养试验改变肠道微生物H2S产生， 通过调节饮食半胱氨酸摄入量来产生微生物H2S。完成拟议目标将导致 到第一个能够长期真实的时间测量肠道微生物H2S产生的可穿戴设备。 这将有助于设计个性化的饮食干预措施，以合理调节肠道微生物的H. 未来的研究寻求解开肠道微生物H2S之间复杂的相互作用， 生产和疾病。