The establishment of methods for cardiomyocyte differentiation with human umbilical cord blood-derived mesenchymal stem cells aimed the application into the treatment of pediatric heart failures

细胞移植是一种很有前途的策略，通过注射体外培养细胞或含有生长因子的培养基来改善器官功能受损。在治疗心力衰竭方面取得了重大进展。然而，由于供体短缺、需要再次移植以及经济成本等原因，儿童心脏病患者仍在等待替代心脏移植的重大治疗。如果治疗的有效性能够得到证实，细胞移植将在这一点上具有优势。儿童期各种原因的严重心力衰竭，包括术后心肌功能不全、心血管衰竭和特发性扩张型心肌病，是难以治疗的，仍然是临床任务。特别是在日本，儿童心脏移植尚未被接受是一个关键问题。因此，我们将重点放在严重心力衰竭患儿的心肌细胞移植上。特别对体外培养的心肌细胞进行了研究，为细胞移植的实际临床应用奠定了基础。本研究旨在促进细胞移植的临床应用。收集间充质干细胞，采用基因芯片分析和Western blot分析其基因表达情况。本研究旨在探索培养或分析人脐带血间充质干细胞的方法，用于安全有效的再生医学。此外，它是。细胞移植需要在未来为受者创造一个护理系统。

The cell transplantation is a promising strategy to improve impaired organ function with injection of ex vivo cultured cells or media containing growth factors. Major advances have been made in the treatment of heart failures. However, pediatric patients suffering from heart disease is still awaiting major treatment which substitute for heart transplantation, because of several reasons such as the shortage of donor, the requirement for retransplantation, and the economic cost. Cell transplantation would have the advantage on this point, if effectiveness of therapy could be proved. Severe heart failures in childhood with variety of causes, including postoperative myocardial insufficiency, cardiovascular failure, and idiopathic dilated cardiomyopathy, are intractable and remain clinical task. Especially in Japan, it is crucial problem that cardiac transplantation in childhood has not been accepted.Thus, we focused on cardiomyocytes transplantation for pediatric patients with severe heart failure. Especially, cardiomyocytes cultured ex vivo were studied for the actual clinical application of cell transplantation. The study aims the clinical application of cell transplantation. Collected mesenchymal stem cells were analyzed for gene expression by Gene chip analysis and Western blot analysis.This study also aimed to exploit the method of cultivation or analysis of human umbilical cord blood-derived mesenchymal stem cells for safe and effective regenerative medicine. In addition, it is. necessary for cell transplantation to generate a care system for recipients in future.