Antitumor effect of gene transfection and DNA vacccine of beta-defensins

本研究探讨了通过基因转导提高β-防御素的表达是否影响鳞状细胞癌的抗肿瘤作用，以及带有β-防御素的DNA疫苗是否影响淋巴瘤的抗肿瘤作用。为观察β-防御素表达增加对鳞状细胞癌的抗肿瘤作用，将携带β-防御素的腺病毒载体导入裸鼠鳞状细胞癌组织中。基因被导入的肿瘤肿块的直径明显小于对照组。结果表明，β-防御素本身可能具有直接的抗鳞癌作用。为了观察带有β-防御素的DNA疫苗对淋巴瘤的抗肿瘤作用，将该疫苗注射到BALB/c小鼠A20淋巴瘤中。接种组小鼠存活率明显高于对照组。结果表明，带有β-防御素的DNA疫苗可能在淋巴瘤的治疗中发挥作用。

The present study examined whether increased expression of beta-defensins by the gene transfection affected antitumor of squamous cell carcinoma (SCC), and whether DNA vaccine with beta-defensins affected antitumor of lymphoma. In order to observe the antitumor effect of increased expression of beta-defensins on SCC, adenovirus vectors with beta-defensin were transfected into SCC tissues in nude mice. The diameter of the tumor mass into which the gene was trandected was significantly smaller than that of the controls. The results indicate that beta-defensins themselves may posses a direct antitumor effect against SCC. In order to observe the antitumor effect of DNA vaccine with beta-defensins on lymphoma, the vaccine was injected into A20 lymphoma inBALB/c mice. The survival rate of the vaccination group was significantly longer than that of the control group. The results indicate that DNA vaccine with beta-defensins may be useful in the treatment of lymphoma.