Molecular Evolutional, Geographical, Population Genetical analyses of Wild SilkMoth including Bombyx mandarina distribute in East Asian region

本研究通过对分布于东亚地区的天蚕等几种野生蚕蛾的部分基因或DNA标记区域的比较，分析了它们在分子水平上的多态性。B。在B中，其突变体由于缺乏或缺乏尿酸在皮肤下的积累而导致半透明皮肤。森。日本对虾的Og基因种类繁多，与B组不同。森。我们对B的线粒体coxl基因进行了分析。Mori，并发现相对于OG基因的序列多样性水平低。碱性磷酸酶（Alp）基因在B中串联复制。森。Alp基因（Alp-m，Alp-s）的核苷酸序列有60%的同源性，但插入区的结构具有高度的多态性，不同菌株间插入/缺失较大。在这项研究中，我们发现Alp基因在B中是重复的。与B中的相似。森，在所有四个宝石。B中的介入区域的结构。B.B.B.提出，目前家蚕品系与野生B的最近共同祖先。在中国，有一种叫“中国人”的东西。从分析Cecropia-ITR-MLE，大多数MLE序列来自B。在日本本岛的古生物组成了一个独特的群（日本型）;另一方面，来自中国和朝鲜标本的序列定居在另一个群（大陆型）。我们已经从韩国和日本的BmTNML基因座中分离出了转座因子（Bmamat 1）的活性类型。B的卵黄原蛋白基因。女同性恋更接近B。桑蚕蛾比其他野生蚕蛾多。

We have analyzed the molecular level of polymorphism in Bombyx mandarina, some other wild silk moth species distribute in wide range of East Asian region by comparing some genes or DNA marker regions among them. B. mori molybdenum cofactor sulfurase gene (og) whose mutants cause translucent skin because of lack or deficiency of accumulation of uric acid under the skin in B. mori. Og genes from Japanese mandarina have wide variety and different from the group of B. mori. We conducted an analysis of mitochondrial coxl gene of B. mori and found low level of sequence diversity relative to that of the og gene. Alkaline phosphatase (Alp) genes are tandemly duplicated in B. mori. The Alp genes (Alp-m, Alp-s) share about 60% identity in the nucleotide sequences, but structure of the intervening region is highly polymorphic with large in/del from strain to strain. In this study we found that the Alp genes were shown to be duplicated in B. mandarina, similarly as in B. mori, in all four gemones. The structure of the intervening region in B. mandarina suggested that the most recent common ancestor of the current silk worm strains and wild B. mandarina existed in China. From the analysis of Cecropia-ITR-MLEs, most of the MLE sequences from B. mandarina in the main island of Japan composed one unique group (Japanese type); on the other hand, the sequences from the Chinese and Korean specimens settled down in another group (Continental type). We have isolated the active type of transposable element (Bmamat1) from the locus of BmTNML of Korean and Japanese mandarina. Vitellogenin genes of B. mandarina are closer to B. mori than other wild silk moth.