The Role of CEACAM1 (CEA-related cell adhesion molecule 1) in vascular morphogenesis and stabilization

血管生成受血管生成激活剂和抑制剂的净平衡调节。细胞粘附分子也参与血管生成的不同阶段，特别是血管形态发生。我们最近鉴定了可溶性CEACAM 1（CEA相关细胞粘附分子1）作为促血管生成因子和VEGF的形态发生效应物。在新生血管的内皮细胞中发现CEACAM 1表达增加，但膜结合CEACAM 1对已知血管生成因子的表达和毛细血管的形态发生的影响仍然未知。本研究的目的是利用以下实验模型探讨膜结合CEACAM 1在血管生成中的作用：1. CEACAM 1在血管内皮和平滑肌细胞中的腺病毒过表达，其随后将用于a）关于已知有效血管生成因子表达的基因阵列分析，B）迁移和体外伤口测定和c）内皮管形成测定。2.将CEACAM 1-腺病毒注射于鸡胚绒毛尿囊膜，研究CEACAM 1基因转染对血管新生和血管渗漏的影响。这些实验将为我们提供关于膜结合CEACAM 1对血管生成的影响的新见解。

Angiogenesis is regulated by a net balance of angiogenic activators and inhibitors. Cell adhesion molecules are also involved in the different stages of angiogenesis, in particular of vascular morphogenesis. We recently charakterized soluble CEACAM1 (CEA-related cell adhesion molecule 1) as a pro-angiogenic factor and morphogenic effector of VEGF. Increased CEACAM1 expression was found in endothelial cells of new blood vessels but the influence of membrane-bound CEACAM1 on the expression of known angiogenic factors and the morphogenesis of capillaries is still unknown. The aim of this study is to explore the role of membran-bound CEACAM1 in angiogenesis using the following experimental models: 1. Adenoviral overexpression of CEACAM1 in vascular endothelial and smooth muscle cells which will be subsequently used a) for gene array analyses regarding the expression of known potent angiogenic factors, b) migration and in vitro wound assays and c) endothelial tube formation assay. 2. Local microinjection of CEACAM1-adenovirus in chicken chorioallantoic membrane to study the effects of gene delivered CEACAM1 in angiogenesis and vascular leakage. These experiments will provide us with new insights concerning the effects of membrane-bound CEACAM1 on the angiogenesis.