Fabrication of Inorganic-Organic Hybrid Membrane with Ion-Channel Function

细胞膜中的离子通道作为信号转导参与生物体内平衡的维持。离子通道作为一种信号换能器，不仅在生物学领域，而且在材料化学领域都具有广泛的应用价值。然而，其作用机制尚不明确。为了阐明这一机理，本研究尝试制备一种具有离子通道功能的人工膜。选择人工离子载体，如冠醚衍生物和杯状衍生物，是因为有报道称它们在单分子中具有离子通道功能，并且这些离子载体的结构修饰是可能的。这些性质对研究其作用机理具有一定的优势。为了提高产率，考察了多种合成路线，并建立了一条比较大规模的合成路线。我们认为，人工膜的性质类似于脂质双层膜，是诱导离子通道功能的必要条件。因此，研究了由磷胆碱、低聚乙二醇和烷烃硫醇组成的膜材料制备人工膜的方法。低聚乙二醇衍生物在氯磷存在下与胆碱反应，得到所需的膜材料。研究发现，用合成的膜材料制备的SAMs对蛋白质的非特异性吸附具有抑制作用。虽然目前还没有制造出具有离子通道功能的人工膜，但这些膜材料有可能成为生物相容性材料，如重组膜。

Ion-channel in cell membrane is known to work as signal transduction to contribute to biological maintenance of homeostasis. The function of ion-channel as a signal transducer is attractive not only in biology but also in material chemistry field because this function is useful for elements of sensing devices. However, its action mechanism is still ambiguous. In order to shed light on this mechanism, fabrication of an artificial membrane with ion-channel function was attempted in this work.Artificial ionophores such as crown ether derivatives and calix derivatives were chosen as they have been reported to show ion-channel function with single molecule and structure modification of those ionophores are possible. Those properties must be of advantage to study the action mechanism. Various synthesis routes were examined in order to improve yields, and a relatively large-scale synthesis route was established.We consider that the nature, similar to lipid bilayer membrane, is necessary for the artificial membrane to induce ion-channel function of an jonophore. Therefore, synthesis of membrane materials consisting of phospho choline, oligo ethylene glycol, and alkane thiol was examined for fabrication of the artificial membrane. The reaction of oligo ethylene glycol derivatives with choline in the presence of phosphoryl chloride afforded the desired membrane materials. It was found that the SAMs fabricated with the synthesized membrane materials show suppressive function for non-specific adsorption of proteins.It is suggested that those membrane materials have a potential for biocompatible materials such as reconstituted membrane although fabrication of an artificial membrane with ion-channel function has not been reached.