Detection and establishment of rapid diagnosis of new badnaviruses in vegetatively propagated plants

收集了印度尼西亚两株表现香蕉条纹病症状的香蕉植株，采用长链pcr和巢式pcr扩增了badnaviravirus属病毒的全长DNA。利用获得的DNA片段进行进一步的南杂交，证实这两株植物都感染了香蕉条纹迈索尔病毒（BSMyV）。在印度尼西亚采集的另一香蕉样本IBoO1对抗bsv抗体反应强烈，DNA分析显示其与BSV-Im具有99%以上的同源性。在印度尼西亚和日本也从菠萝中发现了坏病毒。在热带多年生型植物中，用bad navvirus通用引物组进行PCR检测，结果显示，绿豆（Epipreminum aureum）、丝兰（yucca gloriosa）、野生姜黄（Curcuma aromatica）、窗叶（Monstera deliciosa var. borsigiana）、kalanche （kalanche blossfeldiana）和咖啡（Coffea arabica）均呈阳性反应。在无性繁殖的凤梨中检测到冲绳Bandvirus (ses)，但在种子培养的凤梨中未检测到该病毒；在种子培养的凤梨中未检测到凤梨粉蚧枯萎相关病毒（clostervirus）。另一方面，虽然属凤梨科，但被检测的古兹曼原虫均未感染坏病毒。

Two banana plants showing banana streak disease symptom in Indonesia were collected and amplification of full length DNA of viruses in Genus Badnavirus were conducted both in long-PCR and nested-PCR. Obtained DNA segments were used for further, southern hybridization to show these two plants were infected by Banana streak Mysore virus (BSMyV) . Another banana sample (IBoO1) collected in Indonesia reacted strongly to antiBSV and by DNA analysis, it was shown to have over 99% homology with BSV-Im. From Pineapples, badnaviruses were also detected in Indonesia and Japan. As for tropical perennial type plants, pothos (Epipreminum aureum) , yucca (Yucca gloriosa) , wild turmeric (cultivated, Curcuma aromatica) , window leaf (Monstera deliciosa var. borsigiana) , Kalanchoe (Kalanchoe blossfeldiana) , and coffee (Coffea arabica) showed positive reaction in PCR using Badnavirus universal primer sets. Pineapples in Okinawa Bandvirus (ses) were detected by PCR in vegetatively propagated ones but not in ones grown from seeds while Pineapple mealy bug wilt associated virus (Closterovirus) were not detected from plants grown from seeds. On the other hand, Guzmania spp. tested were all free from Badnavirus infection though they are in Bromeliaceae.