Analysis of mitochondrial DNA in brain tissue in the case of sudden death using micro-dissection system

在体液中，如血液和尿液，很明显，血液和尿液水平密切相关，除了一个肾脏疾病的情况下，检查了铜，铅，镉，锌，镓，汞，锑，砷的水平分布。血中常见的元素为镉，铅含量较少，血、尿中砷、汞、锑等有害元素含量较高，提示有一定的保健功能。此外，镓在体液中的浓度比较高，很可能与基本的生命功能有关。铜在体液中的浓度比较高，比较有趣，因为在受影响的循环患者中，铜的浓度似乎比正常人高，因此被认为是血液循环的必需元素。在脑组织中观察到异常的情况下，其揭示了铜积累大的变化，并且在这种情况下线粒体DNA的异质点突变是多重的，并且很明显，这对老年人来说更大。正常人脑组织线粒体DNA异质性点突变发生率约为0.5%，肾脏病患者有镉蓄积的倾向，但肾脏病患者脑组织病理改变程度明显加重。在肝脏疾病患者中，铜蓄积往往伴随着某种病理变化，但很明显与铅蓄积无关。

In body fluid such as the blood and the urine, it became clear that blood and the urinary level was closely related to except a case of the renal disease examined about a level distribution of copper, lead, cadmium, zinc, gallium, mercury, antimony, arsenic. The element which was usually not seen was cadmium, and comparatively little element was lead in the blood.Noxious element, such as arsenic and mercury and the antimony, are comparatively included in high concentration in blood and urine, and some kind of useful functions is to be suggested. Also, the gallium are included in comparative high concentration in body fluid, and it is likely that it is associated with a basic life function.Included with relatively high concentration, and it is comparatively interesting in body fluid, the copper considered to be essential element for blood circulation because it seems to be included high concentration than a normal human in the affected patient of circulation.Also, in the case that abnormality was seen in brain tissue, it revealed the change that copper accumulation was big, and heteroplasmic point mutation of the mitochondrial DNA were multiplexed in such case, and it became clear that this grew bigger to an elderly person. As for heteroplasmic point mutations of the mitochondrial DNA were produced at the frequency of about 0.5% from the brain analysis of the healthy human.The cadmium tended to be accumulated in the patient of renal disease, but it became clear for such a case that the degree of the cerebral pathologic change was bigger at the same time. The copper accumulation tended to be accompanied with some kind of pathological changes in a patient with hepatic disorder, but it became clear not to be associated with the accumulation of the lead.