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Racial discrimination by direct sequencing of human mitochondrial DNA

人类线粒体 DNA 直接测序的种族歧视

基本信息

批准号：
08670484
负责人：
HONDA Katsuya
金额：
$ 1.41万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670484/
关键词：
mitochondrial DNA Control region Asian population Sequence rariation DNA typing chelex extraction ミトコンドリア DNA 遺伝的多型人種シークエンス変異アジア人人類遺伝学

项目摘要

Hypervariable region of the human mitochondrial DNA was sequenced from 200 samples in several asian populations (Japan, China, Mongolia, Myanmar, Taiwan, Malaysia, and Bangladesh). DNA were extracted from 200 salivas in solution of 5% chelex. PCR were performed using primers L15996 and H408. The sequence variants of the hypervariable regions were detected by fluorescence with a DNA-sequencer (ABI PRISM ; model 377) after cycle sequencing reaction using dye-primer method. We identified distribution and frequency of sequence variations and selected 12 nucleotide in 35bp of the highest variable regions (16158-16192). In these sequence, we devided major three types and three variants types. Frequency of each types were different among asian populations. In Japanese, Anderson's basic type was the most frequent of 65.7%. Unique variation were encountered in in Malaysian and Myanmar populations.We are investigating of true polymorphic site and frequency of polymorphism with realible method. As a results, our findings show almost similar polymorphic pattern was found within Asian population. Then we can grouped major 5 types of mtDNA.Also, we can find that mt-DNA sequencing is not always highly discriminative among the same populations, but is more informative between people from different racial origins.

人类线粒体DNA高变区的测序来自几个亚洲人群（日本，中国，蒙古，缅甸，台湾，马来西亚和孟加拉国）的200个样本。用5%Chelex提取200份唾液DNA。使用引物L15996和H408进行PCR。在使用染料引物法的循环测序反应后，用DNA测序仪（ABI PRISM ;型号377）通过荧光检测高变区的序列变体。我们确定了序列变异的分布和频率，并在最高可变区（16158-16192）的35 bp处选择了12个核苷酸。在这些序列中，我们将其分为三个主要类型和三个变异类型。亚洲人群中各类型的频率不同。日本人以安德森氏基本型最常见，占65.7%。在马来西亚和缅甸群体中发现了独特的变异，我们正在用可靠的方法调查多态性位点和多态性频率。结果表明，在亚洲人群中发现了几乎相似的多态性模式。同时，我们还发现，mtDNA测序在同一人群中并不总是具有很高的区分性，但在不同种族之间的信息量更大。