Racial discrimination by direct sequencing of human mitochondrial DNA

点击翻译按钮获取中文摘要

Hypervariable region of the human mitochondrial DNA was sequenced from 200 samples in several asian populations (Japan, China, Mongolia, Myanmar, Taiwan, Malaysia, and Bangladesh). DNA were extracted from 200 salivas in solution of 5% chelex. PCR were performed using primers L15996 and H408. The sequence variants of the hypervariable regions were detected by fluorescence with a DNA-sequencer (ABI PRISM ; model 377) after cycle sequencing reaction using dye-primer method. We identified distribution and frequency of sequence variations and selected 12 nucleotide in 35bp of the highest variable regions (16158-16192). In these sequence, we devided major three types and three variants types. Frequency of each types were different among asian populations. In Japanese, Anderson's basic type was the most frequent of 65.7%. Unique variation were encountered in in Malaysian and Myanmar populations.We are investigating of true polymorphic site and frequency of polymorphism with realible method. As a results, our findings show almost similar polymorphic pattern was found within Asian population. Then we can grouped major 5 types of mtDNA.Also, we can find that mt-DNA sequencing is not always highly discriminative among the same populations, but is more informative between people from different racial origins.